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微透析/液相色谱-质谱联用筛选黄芩中α-葡萄糖苷酶抑制剂
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庞博,王倩倩,刘舒,刘志强,宋凤瑞 1.中国科学院长春应用化学研究所长春质谱中心&吉林省中药化学与质谱重点实验室吉林长春1300222.中国科学技术大学应用化学与工程学院安徽合肥2300263.南开大学药物化学生物学国家重点实验室天津300457 
基金项目:国家自然科学基金项目(21673219,81373952)
中文摘要:应用微透析/液相色谱-质谱联用法筛选黄芩中α 葡萄糖苷酶抑制剂,并对筛选出的具有酶抑制活性的化合物进行结构鉴定。实验采用迪马C18色谱柱(150 mm×46 mm,5 μm),以05%醋酸水-乙腈溶液为流动相,梯度洗脱,流速05 mL/min,柱温30 ℃,以正离子电喷雾质谱模式采集数据,从黄芩中筛选出10种潜在的α 葡萄糖苷酶抑制剂,10种化合物与α 葡萄糖苷酶的结合率为3618%~5271%。该方法可快速、有效地筛选黄芩中的α 葡萄糖苷酶的抑制剂,同时可为从其他中药中筛选酶抑制剂提供方法学借鉴。
中文关键词:黄芩  α 葡萄糖苷酶  微透析  HPLC-MSn  抑制剂
 
Screening of α Glucosidase Inhibitors in Scutellaria Baicalensis by Liquid Chromatography-Mass Spectrometry with Microdialysis
Abstract:A liquid chromatography-mass spectrometry with microdialysis was used to screen potential α glucosidase inhibitors in the extracts of Scutellaria baicalensis and identify the structures of those bioactive components screened.The chromatographic separation was carried out on a Dikma C18 column(150 mm×46 mm,5 μm) by gradient elution with 05% acetic acid solution-acetonitrile as mobile phases at a flow rate of 05 mL/min and a column temperature of 30 ℃.Mass spectrometry analysis and data acquisition were conducted in the positive ion mode.In this work,ten potential α glucosidase inhibitors in Scutellaria baicalensis were found and identified simultaneously.The binding degrees of 10 compounds with α glucosidase ranged from 3618% to 5271%.With the advantages of rapidness,high throughput and effectiveness,this method could screen α glucosidase inhibitors in Scutellaria baicalensis and provide valuable information for screening α glucosidase inhibitors from other traditional Chinese medicines.
Key Words:Scutellaria baicalensis  α glucosidase  microdialysis  HPLC-MSn  inhibitor
引用本文:庞博,王倩倩,刘舒,刘志强,宋凤瑞.微透析/液相色谱-质谱联用筛选黄芩中α-葡萄糖苷酶抑制剂[J].分析测试学报,2020,39(1):157-161.
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