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在线固相萃取净化-高效液相色谱法测定人血浆中的霉酚酸
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作者单位
许泽君,景霞,孙芳,吉超,杨新磊 1.南京医科大学附属儿童医院2.安捷伦科技(中国)有限公司 
基金项目:江苏省药学会—奥赛康医院药学基金科研项目(A201810);南京市药学会-常州四药医院药学科研基金(2019YX003)
中文摘要:建立了在线固相萃取净化-高效液相色谱法联用(Online SPE-HPLC)测定人血浆中霉酚酸浓度的分析方法。联用系统以Capcell PAK MF Ph-1为净化柱,Poroshell 120- EC-C18为分析柱,血浆样品经甲醇沉淀蛋白后,直接进样分析。结果表明,霉酚酸在0.20~50.00 μg/mL范围内线性关系良好,相关系数(r)为0.999 8,检出限和定量下限分别为0.07、0.20 μg/mL。在0.39、25.00、50.00 μg/mL 3个质量浓度下的平均回收率为96.2%~105%,日内和日间相对标准偏差(RSD)分别为2.6%~3.1%和2.9%~3.3%。该法操作简便、快速,可用于人血浆中霉酚酸浓度的分析。
中文关键词:霉酚酸  血浆  在线固相萃取-高效液相色谱法(Online SPE-HPLC)
 
Determination of Mycophenolic Acid in Human Plasma by High Performance Liquid Chromatography with Online Solid Phase Extraction Purification
Abstract:An online solid phase extraction-high performance liquid chromatographic(Online SPE-HPLC) method was developed for the determination of mycophenolic acid in human plasma.Plasma samples were directly injected into the online SPE-HPLC system after simple protein precipitation.Capcell PAK MF Ph-1 was employed as an online SPE column and Poroshell 120 EC-C18 was employed as an analysis column.Results showed that there was a good linear relationship for mycophenolic acid in the concentration range of 0.20-50.00 μg/mL,with a correlation coefficient(r) of 0.999 8.The limit of detection(LOD) and limit of quantitation(LOQ) were of 0.07 μg/mL and 0.20 μg/mL,respectively.The spiked recoveries for mycophenolic acid in human plasma at three spiked levels of 0.39,25.00 and 50.00 μg/mL ranged from 96.2% to 105%,with intra assay relative standard deviations(intra-RSD) of 2.6%-3.1% and inter-assay relative standard deriation(inter-RSD) of 2.9%-3.3%.The method was rapid and simple,and was suitable for the determination of mycophenolic acid concentrations in human plasma.
Key Words:mycophenolic acid  human plasma  online solid phase extraction-high performance liquid chromatography(online SPE-HPLC)
引用本文:许泽君,景霞,孙芳,吉超,杨新磊.在线固相萃取净化-高效液相色谱法测定人血浆中的霉酚酸[J].分析测试学报,2020,39(1):162-166.
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