| 在线固相萃取净化-高效液相色谱法测定人血浆中的霉酚酸 |
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| 基金项目:江苏省药学会—奥赛康医院药学基金科研项目(A201810);南京市药学会-常州四药医院药学科研基金(2019YX003) |
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| 中文摘要:建立了在线固相萃取净化-高效液相色谱法联用(Online SPE-HPLC)测定人血浆中霉酚酸浓度的分析方法。联用系统以Capcell PAK MF Ph-1为净化柱，Poroshell 120 EC-C18为分析柱，血浆样品经甲醇沉淀蛋白后，直接进样分析。结果表明，霉酚酸在020~5000 μg/mL范围内线性关系良好，相关系数(r)为0999 8，检出限和定量下限分别为007、020 μg/mL。在039、2500、5000 μg/mL 3个质量浓度下的平均回收率为962%~105%，日内和日间相对标准偏差(RSD)分别为26%~31%和29%~33%。该法操作简便、快速，可用于人血浆中霉酚酸浓度的分析。 |
| 中文关键词:霉酚酸 血浆 在线固相萃取-高效液相色谱法(Online SPE-HPLC) |
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| Determination of Mycophenolic Acid in Human Plasma by High Performance Liquid Chromatography with Online Solid Phase Extraction Purification |
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| Abstract:An online solid phase extraction-high performance liquid chromatographic(Online SPE-HPLC) method was developed for the determination of mycophenolic acid in human plasma.Plasma samples were directly injected into the online SPE-HPLC system after simple protein precipitation.Capcell PAK MF Ph-1 was employed as an online SPE column and Poroshell 120 EC-C18 was employed as an analysis column.Results showed that there was a good linear relationship for mycophenolic acid in the concentration range of 020-5000 μg/mL,with a correlation coefficient(r) of 0999 8.The limit of detection(LOD) and limit of quantitation(LOQ) were of 007 μg/mL and 020 μg/mL,respectively.The spiked recoveries for mycophenolic acid in human plasma at three spiked levels of 039,2500 and 5000 μg/mL ranged from 962% to 105%,with intra assay relative standard deviations(intra RSD) of 26%-31% and inter assay relative standard deriation(inter RSD) of 29%-33%.The method was rapid and simple,and was suitable for the determination of mycophenolic acid concentrations in human plasma. |
| Key Words:mycophenolic acid human plasma online solid phase extraction-high performance liquid chromatography(online SPE-HPLC) |
| 引用本文：许泽君，景霞，孙芳，吉超，杨新磊.在线固相萃取净化-高效液相色谱法测定人血浆中的霉酚酸[J].分析测试学报,2020,39(1):162-166. |
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