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PSMA@Ag复合微球的制备及其在MALDI-MS分析小分子化合物中的应用研究
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作者单位
谢斌,李万万 上海交通大学材料科学与工程学院 
基金项目:国家自然科学基金资助项目( 81671782,81971704)
中文摘要:基质辅助激光解吸电离质谱(MALDI-MS)作为一种有力的分析手段,在生物分子分析中有着广泛的应用,但很难应用于分子量小于500的待测物的分析。该文利用聚多巴胺修饰还原法制备了核壳结构的聚苯乙烯-马来酸酐共聚物@银纳米壳层(PSMA@Ag)复合微球。采用傅立叶红外光谱法验证了聚多巴胺(PDA)的成功修饰。结合扫描电子显微镜(SEM)和紫外-可见光谱(UV-Vis)分析结果,发现Ag纳米壳层成功地包覆在PSMA微球的表面。将制备的PSMA@Ag复合微球作为辅助基质直接应用于MALDI-MS,成功地从0.5 μL待测物样品中检测到2 pmol脯氨酸(Mw=115)和1 pmol丝氨酸(Mw=105)。研究结果证明PSMA@Ag微球对MALDI的离子化过程有促进作用,为解决MALDI-MS在分析小分子待测物时背景噪声过大,信号无法分辨的问题提供了一个有效途径。
中文关键词:核壳结构  多巴胺    基质辅助激光解吸电离质谱
 
Fabrication of PSMA@Ag Composite Microbeads and Their Application in Small Molecular Analysis Based on MALDI-MS Platform
Abstract:As a powerful analysis approach,matrix assisted laser desorption/ionization mass spectrometry(MALDI-MS) has attracted considerable attention in biomolecular analysis.However,this approach could hardly be used in the detection for samples with molecular mass lower than 500.In this work,poly (styrene-co-maleic anhydride) microbead@silver nanoshell(PSMA@Ag) composite microbeads with core-shell structure were synthesized using a polydopamine modification method.FT-IR results demonstrated the successful coating of polydopamine(PDA).SEM images along with UV-Vis results indicated that Ag nanoparticles were uniformly modified onto the surface of PSMA microbeads.The prepared PSMA@Ag microbeads were directly applied in matrix assisted laser desorption/ionization mass spectrometry,successfully detecting 2 pmol proline (Mw=115) and 1 pmol serine(Mw=105) in 0.5 μL sample,respectively.This work verified the positive effect of PSMA@Ag microbeads in MALDI-MS laser desorption/ionization process,and provided a promising route for MALDI analysis of the biomolecules with low molecular mass.
Key Words:core-shell structure  dopamine  silver  matrix assisted laser desorption/ ionization mass spectrometry (MALDI-MS)
引用本文:谢斌,李万万.PSMA@Ag复合微球的制备及其在MALDI-MS分析小分子化合物中的应用研究[J].分析测试学报,2020,39(3):295-300.
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