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基于Au@Ag纳米颗粒的表面增强拉曼检测血浆中的法莫替丁
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作者单位
臧颖超,朱慧敏,范夏琼,张志敏,卢红梅 中南大学化学化学工学院 
基金项目:国家自然科学基金资助(21675174)
中文摘要:该文开发了一种基于Au@Ag纳米颗粒(Au@AgNPs)快速检测法莫替丁(FMD)的表面增强拉曼光谱(SERS)方法。通过种子介导法合成了Au@AgNPs,再采用场电子发射显微镜(SEM)、透射电子显微镜(TEM)、紫外可见吸收光谱(UV-Vis)和X射线衍射仪(XRD)对Au@AgNPs进行表征,以罗丹明6(RG6)为分子探针对合成的Au@AgNPs的SERS性能进行了测试。结果显示,合成的Au@AgNPs形貌均匀,具有良好的增强效果,增强因子为6.66×105,将其用于法莫替丁标准溶液的SERS检测,在0.5~50 μmol/L范围内法莫替丁浓度的对数和1 540 cm-1处峰强度的对数具有较好的线性关系,相关系数(r2)为0.978,检出限(LOD)为4.18×10-8 mol/L。在同样的条件下对稀释血浆中的法莫替丁进行了SERS检测,LOD为1.11×10-7 mol/L,加标回收率为91.6%~122%,相对标准偏差为6.6%~12%,表明该方法快速、灵敏,在生物样本检测方面具有潜在的应用价值。
中文关键词:表面增强拉曼光谱;核壳金银纳米粒子;法莫替丁  血浆
 
Detection of Famotidine in Plasma by Surface Enhanced Raman Spectroscopy Based on Au@Ag Nanoparticles
Abstract:In this paper,a method of surface enhanced Raman spectroscopy(SERS) based on core shell Au@Ag nanoparticles(Au@AgNPs) was developed for the rapid detection of famotidine(FMD).The Au@AgNPs were synthesized by seed growth method,and then characterized by SEM,TEM,UV-Vis spectrometry and XRD.Rhodamine 6(RG6) was used as the molecular probe to evaluate the SERS performance of Au@AgNPs.The synthesized Au@AgNPs has a uniform morphology and a good enhancement effect,with an enhancement factor of 6.66×105.The Au@AgNPs were used for SERS detection of FMD standard solution.Results showed that there was a good linear relationship between logarithm of concentration and logarithm of FMD peak intensity at 1 540 cm-1 in the range of 0.5-50 μmol/L,with a correlation coefficient(r2) of 0.978 and a detection limit(LOD) of 4.18×10-8 mol/L.Under the same conditions,Au@AgNPs were used for SERS detection of FMD in diluted plasma,with a LOD as low as 1.11×10-7 mol/L.The spiked recoveries were in the range of 91.6%-122% with relation standard deviations of 6.6%-12%.The experimental results demonstrated that this method is fast and sensitive,and has a potential application value in detection of biological sample.
Key Words:surface enhanced Raman spectroscopy(SERS)  core shell Au@Ag nanoparticles  famotidine  plasma
引用本文:臧颖超,朱慧敏,范夏琼,张志敏,卢红梅.基于Au@Ag纳米颗粒的表面增强拉曼检测血浆中的法莫替丁[J].分析测试学报,2020,39(6):779-783.
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