•  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
期刊检索


检索
检索项:
检索词:
总目录
  • WCSB9︱2019(第九届)世界采样和混样大会 查看全文>>
  • 关于召开第十五届全国青年分析测试学术报告会的通知 查看全文>>
友情链接
基于FRET效应的碳量子点/银纳米粒子荧光探针测定西咪替丁的研究
    点此下载全文
作者单位
张洁,张越诚,李承佳,马红燕,李恒,辛程宏,李昊阳 延安大学化学与化工学院延安市分析技术与检测重点实验室 
基金项目:延安大学科研计划青年项目(YDQ2018-17);延安大学大学生创新创业训练项目(DC2019-31)
中文摘要:以天然水果杨桃为唯一原料,由水热法直接合成了荧光强度高、发光性能良好的杨桃碳量子点( CB-CQDs),用紫外-可见光谱(UV-Vis)、红外光谱(FTIR)、透射电镜(TEM)、荧光光谱(FL)分别表征了CB-CQDs形貌和光学特性。研究发现,在pH 60的伯瑞坦-罗宾森(BR)缓冲溶液中,向CB-CQDs溶液中加入银纳米粒子(AgNPs)后,AgNPs与CB-CQDs之间产生荧光共振能量转移(FRET)效应(CB-CQDs和AgNPs分别为荧光供体与受体),使CB-CQDs荧光猝灭,荧光信号“关闭”;于该体系中加入西咪替丁(Cimetidine,CMT)后可使猝灭的荧光强度部分恢复,荧光信号重新“打开”,据此可实现CMT的定量测定。基于以上原理提出了以CB-CQDs/AgNPs为荧光探针测定CMT的荧光分析新方法,并对反应机理进行了讨论。在优化条件下,CMT浓度在9.0×10-8~1.0×10-6 mol/L范围内与CB-CQDs在λex/λem=319 nm/397 nm处的荧光恢复值(ΔIF)呈良好的线性关系,相关系数(r)为0.998 6,检出限(3σ/k)为1.9×10-8 mol/L。将该方法用于样品中CMT的测定,结果满意。
中文关键词:杨桃  碳量子点  银纳米粒子  荧光共振能量转移  西咪替丁
 
Determination of Cimetidine Using a Fluorescent Probe Based on Fluorescence Resonance Energy Transfer Effect between Carbon Quantum Dots and Silver Nanoparticles
Abstract:In this paper,a fluorescent probe for the dectection of cimetidine was fabricated based on fluorescence resonance energy transfer effect(FRET) between carambola carbon quantum dots(CB-CQDs) and silver nanoparticles(AgNPs).The CB-CQDs with high fluorescence intensity and good luminescence performance were synthesized by hydrothermal method using natural fruit carambola as the only raw material,which morphology and properties were characterized by ultraviolet-visible spectroscopy(UV-Vis),infrared spectroscopy(FTIR),transmission electron microscopy(TEM) and fluorescence spectrometry(FL),respectively.It was found that,when AgNPs was added into a pH 6.0 Britton-Robinson buffer solution containing CB-CQDs,the fluorescence signal of CB-CQDs at λex/λem=319 nm/397 nm was gradually quenched through the FRET effect,in which CB-CQDs and AgNPs acted as fluorescence donor and fluorescence acceptor,respectively.While in the presence of cimetidine,the fluorescence signal of CB-CQDs was restored and fluorescence signal “turned on” again,forming an “off-on” cimetidine sensor,and the basic mechanism was further discussed.Under the optimized conditions,there was a good linear relationship between fluorescence recovery value(ΔIF) and cimetidine concentration in the range of 9.0×10-8-1.0×10-6 mol/L,with a correlation coefficient(r) of 0.998 6.The detection limit was calculated to be 1.9×10-8 mol/L based on the 3σ/k principle.This method was successfully applied to the detection of cimetidine in real samples with satisfactory results.
Key Words:carambola  carbon quantum dots  silver nanoparticles  fluorescence resonance energy transfer  cimetidine
引用本文:张洁,张越诚,李承佳,马红燕,李恒,辛程宏,李昊阳.基于FRET效应的碳量子点/银纳米粒子荧光探针测定西咪替丁的研究[J].分析测试学报,2020,39(7):894-899.
摘要点击次数: 79
全文下载次数: 138
查看全文  查看/发表评论  下载PDF阅读器