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高效液相色谱-串联质谱法测定睫毛增长液化妆品中的比马前列素
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潘晨,许勇,韩晶,彭兴盛,郑荣 上海市食品药品检验所国家药监局化妆品监测评价重点实验室 
基金项目:上海市药品监督管理局重大资助项目(ZD202003)
中文摘要:建立了高效液相色谱-串联质谱法(HPLC-MS/MS)测定睫毛增长液化妆品中比马前列素含量的分析方法。样品经50%(体积分数)甲醇溶液提取,Agilent Poroshell 120 EC-C18柱(4.6 mm×100 mm,2.7 μm)分离,以5 mmol·L-1乙酸铵水溶液(含0.02%甲酸)-乙腈(体积比55 ∶45)为流动相等度洗脱,流速0.3 mL·min-1,采用电喷雾正离子源(ESI+),多反应监测(MRM)扫描方式检测,外标法定量。结果表明,比马前列素在0.5~100 ng·mL-1质量浓度范围内线性良好,相关系数(r2)为0.998 2,在低、中、高3个加标水平下的平均回收率分别为98.6%、101%和99.2%,相对标准偏差(RSD)分别为2.4%、2.1%、1.2%,检出限(LOD,S/N=3)为0.015 μg·g-1,定量下限(LOQ,S/N=10)为0.05 μg·g-1。该方法快速简便、灵敏度高,适用于市售睫毛增长液化妆品中比马前列素的快速准确检测。
中文关键词:比马前列素  睫毛增长  化妆品  高效液相色谱-串联质谱法(HPLC-MS/MS)
 
Determination of Bimatoprost in Eyelash Enhancing Cosmetics Serums by High Performance Liquid Chromatography-Tandem Mass Spectrometry
Abstract:A high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS) method was established for the determination of bimatoprost in eyelash enhancing cosmetics serums.The samples were extracted with 50%(by vloume) methanol,then separated on an Agilent Poroshell 120 EC-C18(4.6 mm×100 mm,2.7 μm) column by isocratic elution,with 5 mmol·L-1 ammonium acetate containing 0.02% formic acid-acetonitrile(55∶45) as mobile phases at a flow rate of 0.3 mL·min-1.The analyte was detected by HPLC-MS/MS with electrospray ionization source in positive ion mode(ESI+) and multiple reaction monitoring(MRM),and quantified by external standard method.Results showed that there existed a good linear relationship for bimatoprost in the range of 0.50-100 ng·mL-1 with a correlation coefficient(r2) of 0.998 2.The limit of detection(LOD,S/N=3) and limit of quantitation(LOQ,S/N=10) were 0.015 and 0.05 μg·g-1,respectively.The recoveries at low,medium and high spiked levels were 98.6%,101% and 99.2%,with relative standard deviations(RSDs) of 2.4%,2.1% and 1.2%,respectively.The method is simple,rapid and high sensitive,and is suitable for the rapid determination of bimatoprost in eyelash enhancing cosmetics serums.
Key Words:bimatoprost  eyelash enhancing  cosmetics  high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)
引用本文:潘晨,许勇,韩晶,彭兴盛,郑荣.高效液相色谱-串联质谱法测定睫毛增长液化妆品中的比马前列素[J].分析测试学报,2020,39(7):917-921.
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