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近红外光谱法快速测定柴胡提取过程中的药效成分
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作者单位
杨越,杨留长,纪晓亮,李易航,徐倩茹,佟海滨 1.温州大学生命与环境科学学院
2.浙江省水环境与海洋生物资源保护重点实验室
3.温州医科大学公共卫生与管理学院 
基金项目:国家自然科学基金资助项目(31900274,81872952);温州市科技计划项目(S20180013,Y20180210);温州大学2020年实验室开放项目(JW20SK46)
中文摘要:利用近红外光谱(NIRS)技术对柴胡提取过程中的药效成分进行快速定量分析。共收集126个柴胡提取液样品,采用紫外-可见分光光度法测定总黄酮和多糖的含量,高效液相色谱法(HPLC)测定柴胡皂苷A及柴胡皂苷D的含量,以透射模式采集提取液的近红外光谱,运用偏最小二乘法(PLS)分别建立了近红外光谱与4种药效指标参考值之间的定量校正模型,并采用不同的预处理方法、光谱波段和主因子数对模型进行优化。结果表明,总黄酮、多糖、柴胡皂苷A和柴胡皂苷D 4种定量模型的近红外预测值与参考值之间的拟合性良好,模型预测精度较高,其中预测集相关系数(RP)均大于0.9;预测集误差均方根(RMSEP)分别为3.46 μg/mL、0.743 mg/mL、1.53 μg/mL、0.406 μg/mL;预测集相对偏差(RSEP)分别为1.65%、8.28%、5.74%、7.52%。该研究证实了NIRS结合PLS可成功应用于监测柴胡提取液中药效成分的含量变化,且方法具有快速、准确、无损和环保的特点。
中文关键词:近红外光谱(NIRS);柴胡;提取过程;药效成分  快速分析
 
Scientific PapersRapid Determination of Active Components in Extraction Process of Bupleuri Radix by Near Infrared Spectroscopy
Abstract:A near infrared spectroscopy(NIRS) was applied to the rapid and quantitative analysis of active components in the extraction process of Bupleuri Radix.Total of 126 extraction samples were selected,of which the contents of total flavonoids and polysaccharide were determined by ultraviolet-visible spectrophotometry,while the saikosaponin A and saikosaponin D were determined by high performance liquid chromatography(HPLC),and their spectra were collected in transmission mode.The quantitative calibration models for above four compounds were established by partial least squares(PLS),which were optimized by using different pretreatment methods,spectral bands and the number of principle components.Results indicated that there existed good fitting of the four quantitative calibration models between predicted values and the reference values,which exhibited high prediction accuracy with correlation coefficients in prediction sets(RP) greater than 0.9.The root mean square errors of prediction(RMSEP) were 3.46 μg/mL,0.743 mg/mL,1.53 μg/mL,0.406 μg/mL for total flavonoids,polysaccharide,saikosaponin A and saikosaponin D,respectively,and the relative standard errors of prediction(RSEP) were 1.65%,8.28%,5.74% and 7.52%,respectively.All the results confirmed that the combination of NIRS and PLS could be successfully applied to the monitoring of active components in the extraction of Bupleuri Radix with the advantages of rapidness,accuracy,non-destruction and environmental friendly.
Key Words:near infrared spectroscopy(NIRS)  Bupleuri Radix  extraction process  active components  rapid analysis
引用本文:杨越,杨留长,纪晓亮,李易航,徐倩茹,佟海滨.近红外光谱法快速测定柴胡提取过程中的药效成分[J].分析测试学报,2020,39(11):1311-1319.
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