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基于质量标志物的当归抗炎功效近红外快速评价
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作者单位
闫孟琳,丛龙飞,张子玥,姜民,聂磊,白钢 1.南开大学药学院药物化学生物学国家重点实验室2.山东大学药学院 
基金项目:天津市科技援助项目(18YFYZCG00060)
中文摘要:该文通过筛选当归中对NF-κB具有抑制活性的质量标志物(Q-marker),建立了一种利用近红外光谱技术(NIRS)快速评价当归抗炎功效的方法。采用UPLC/Q-TOF技术结合NF-κB双荧光素酶报告基因对当归中具有NF-κB抑制活性的成分进行筛选,并通过单体、整体及等效性验证确立了关键Q marker。以UPLC分别对多批当归药材中的Q-marker进行含量测定,建立其NIRS定量模型,并进一步将Q-marker的含量与当归的NF-κB抑制活性相关联,构建“量-效”拟合函数。结果表明,绿原酸(X1)、洋川芎内酯I(X3)和Z-藁本内酯(X4)为NF-κB抑制活性的关键Q-marker,其含量波动与整体抗炎活性(Y)的变化相吻合,符合拟合方程:Y=16.13-1484X1+7.981X3+0.112 6X4,且基于NIRS的预测效果良好。该方法通过整合分析实现了基于关键Q-marker的当归药材抗炎功效的快速评价,为中药材品质的快速分析提供了新的研究范式和解决方案。
中文关键词:当归  质量标志物  NF-κB  近红外技术  质量评价
 
Rapid Evaluation of Anti inflammatory Efficacy of Angelica sinensis(Oliv.) Diels by Near Infrared Spectroscopy Based on Quality Markers
Abstract:By screening the quality markers(Q-markers) of NF-κB inhibitory active ingredients,a method of near infrared spectroscopy(NIRS) was established for the rapid evaluation of anti inflammatory effect of Angelica sinensis(AS).The NF-κB inhibitory active ingredients in AS were screened by UPLC/Q-TOF combined with NF-κB double luciferase reporter gene assay system.To establish the Q-markers,the inhibitory effects were further evaluated using active ingredients,AS extract and equivalence verification.The Q-markers in multi batches of AS were quantitatively analyzed by UPLC and NIRS,and then the NIRS fitting algorithm was established.Meanwhile,the relationship between NF-κB inhibitory holistic activity of AS extract and the content of Q-markers was investigated.Finally,the prediction model for anti-inflammatory efficacy of AS was constructed based on Q-markers check analysis via NIRS technique.The screening results showed that chlorogenic acid(X1),senkyunolide I(X3) and Z-ligustilide(X4) in AS had significant NF-κB inhibitory effects,and the change of content was consistent with the capacity of NF-κB inhibitory action of AS extracts,which satisfied the following functions:Y=16.13-14.84X1+7.981X3+0.112 6X4.In addition,the measured values from NIRS simulation method for the Q-markers detection showed a good correlation with the predicted values.The developed method realized the rapid evaluation on the anti-inflammatory effects of AS based on Q-markers,and provided a new research paradigm and solution for the rapid quality evaluation of traditional Chinese medicine.
Key Words:Angelica sinensis(Oliv.) Diels  quality markers  NF-κB  near infrared spectroscopy(NIRS)  quality evaluation
引用本文:闫孟琳,丛龙飞,张子玥,姜民,聂磊,白钢.基于质量标志物的当归抗炎功效近红外快速评价[J].分析测试学报,2020,39(11):1320-1326.
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