•  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
期刊检索


检索
检索项:
检索词:
总目录
  • WCSB9︱2019(第九届)世界采样和混样大会 查看全文>>
  • 关于召开第十五届全国青年分析测试学术报告会的通知 查看全文>>
友情链接
基于DNA四面体纳米结构探针的电化学生物传感器检测DNA甲基化
    点此下载全文
作者单位
韩苗苗,王萍,席守民 河南科技大学基础医学院 
基金项目:国家自然科学基金项目(61701171);河南省高等学校重点科研项目计划(18A180012)
中文摘要:该文以DNA四面体纳米结构探针(TSP)为捕获探针,将辣根过氧化物酶标记的IgG抗体结合在纳米金颗粒表面(AuNPs-IgG-HRP)作为信号分子,构建了一种新型DNA甲基化电化学传感器。利用一步热变性法组装成TSP后,通过Au—S键固定在修饰纳米金颗粒的金电极表面,经过靶标DNA杂交、5-甲基胞嘧啶(5-mc)抗体及AuNPs-IgG-HRP结合后,用差分脉冲伏安法(DPV)进行检测。采用循环伏安法(CV)和电化学阻抗谱(EIS)对修饰电极的构建过程进行电化学表征。探究了杂交时间、5-mc抗体浓度、IgG-HRP加入体积、氢醌(HQ)和过氧化氢(H2O2)浓度对传感器的影响。在最佳条件下,该传感器对甲基化DNA的线性响应范围为1.0×10-15~1.0×10-10 mol/L,检出限(S/N=3)为4.4×10-16 mol/L。该传感器具有良好的选择性和稳定性,为DNA甲基化检测提供了新方法。
中文关键词:DNA四面体纳米结构探针  电化学传感器  DNA甲基化  纳米金  抗体
 
An Electrochemical Biosensor Based on a DNA Tetrahedral Nanostructure Probe for Detection of DNA Methylation
Abstract:In this study,a novel electrochemical sensor was constructed for the detection of DNA methylation,with a DNA tetrahedral nanostructure probe(TSP) as capture probe and horseradish peroxidase-labeled IgG antibody bound to the surface of gold nanoparticles (AuNPs-IgG-HRP) as a signal molecule.After being assembled into TSP by the one step thermal denaturation method,the probe was fixed on the surface of AuNPs/AuE electrode through Au-S bond.Experienced the target DNA hybridization,5-methylcytosine (5-mc) antibody and AuNPs-IgG-HRP conjugation,the electrochemical signal was obtained by differential pulse voltammetry(DPV).The construction process of the modified electrode was characterized by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS).Effects of hybridization time,5-mc antibody concentration,addition volume of IgG-HRP,concentrations of hydroquinone(HQ) and hydrogen peroxide(H2O2) on the sensor were explored.Under the optimal conditions,the biosensor exhibited a good linear response toward the methylated DNA in the range of 1.0×10-15-1.0×10-10 mol/L,with a detection limit (S/N=3) of 4.4×10-16 mol/L.The biosensor could provide a new method for the detection of DNA methylation with good selectivity and stability.
Key Words:DNA tetrahedral nanostructure probe  electrochemical sensor  DNA methylation  gold nanoparticles  antibody
引用本文:韩苗苗,王萍,席守民.基于DNA四面体纳米结构探针的电化学生物传感器检测DNA甲基化[J].分析测试学报,2020,39(12):1466-1472.
摘要点击次数: 238
全文下载次数: 353
查看全文  查看/发表评论  下载PDF阅读器