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基于液相色谱-质谱法的不同花期金银花代谢组学分析
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作者单位
李丽丽,李月,卢恒,王晓 齐鲁工业大学(山东省科学院)山东省分析测试中心山东省大型精密分析仪器应用技术重点实验室 
基金项目:国家自然科学基金项目(21904080);山东省科学院青年基金项目(2019QN011)
中文摘要:基于液相色谱-质谱(LC-MS)建立了金银花代谢组学分析方法,优化得到最佳提取试剂为甲醇-水(体积比3∶1),并对方法的重复性和精密度进行了考察。结果表明方法的稳定性良好,满足代谢组学分析的要求。通过质量数、保留时间和二级质谱数据对金银花中的初生和次生代谢物进行了定性分析,共鉴别出157个代谢物,包括氨基酸、核苷、脂肪酸、脂质等初生代谢物以及酚酸、黄酮、环烯醚萜苷等次生代谢物。不同花期的金银花代谢组学分析发现,次生代谢物酚酸类、黄酮类和环烯醚萜苷类的含量随着花期的增加而显著下降。初生代谢物中,大部分氨基酸、核苷、氧化脂肪酸的含量显著下降,而大部分的溶血磷脂酰胆碱(Lyso PC)、溶血磷脂酰乙醇胺(Lyso PE)和脂肪酸的含量显著上升。该研究结果可为金银花药效成分代谢调控机制的深入研究及后期种植采收提供理论指导。
中文关键词:金银花  代谢组学  液相色谱-质谱(LC-MS)  初生代谢物  次生代谢物  花期
 
Metabolomics Analysis of Lonicera japonica in Different Flowering Stages Based on Liquid Chromatography-Mass Spectrometry
Abstract:An analytical method for the metabolomics of Lonicera japonica was established based on liquid chromatography-mass spectrometry(LC-MS).The extraction solution was optimized as a mixture solution of methanol and water(3∶1,by volume).The repeatability and precision of the method were investigated.It was found that the method could meet the requirements for metabolomics analysis with good stability.The primary and secondary metabolites in Lonicera japonica were identified by accurate mass,retention time and MS/MS data.A total of 157 metabolites were identified,including amino acids,nucleosides,fatty acids,lipids,phenolic acids,flavonoids and iridoid glycosides,etc.According to the metabolomics analysis of Lonicera japonica in different flowering stages,it was found that the secondary metabolites of phenolic acid,flavonoids and iridoid glycosides decreased significantly with the process of flowering stages.For the primary metabolites,the contents of most amino acids,nucleosides and oxidized fatty acids decreased significantly,while the contents of most Lyso PC,Lyso PE and fatty acids increased significantly.The results obtained provide theoretical guidances for study on the metabolic mechanism of medicinal components and the late planting and harvesting of Lonicera japonica.
Key Words:Lonicera japonica  metabolomics  liquid chromatography-mass spectrometry(LC-MS)  primary metabolites  secondary metabolites  flowering stages
引用本文:李丽丽,李月,卢恒,王晓.基于液相色谱-质谱法的不同花期金银花代谢组学分析[J].分析测试学报,2020,39(12):1501-1507.
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