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基于纳米金/聚赖氨酸修饰的丝网印刷电极免疫传感器对双酚A的灵敏检测
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何祖宇,李普旺,周闯,王超,吕明哲,宋书会,刘运浩,杨子明 1.中国热带农业科学院南亚热带作物研究所海南省热带园艺产品采后生理与保鲜重点实验室2.中国热带农业科学院农产品加工研究所 
基金项目:海南省自然科学基金项目(219QN294);云南省李普旺专家工作站项目(202005AF150007);广东省基础与应用基础研究基金项目(2018A030313109,2019A1515010714);中国热带农业科学院基本科研业务费专项资金(1630122020004,1630122017009);湛江市科技发展专项资金竞争性分配项目(2018A02015)
中文摘要:该文采用一次性丝网印刷碳电极为基底,利用电沉积法制备了聚L 赖氨酸/纳米金修饰电极,构建了双酚A(BPA)电化学免疫传感器。结果表明,当抗原和第一抗体的稀释倍数为1 000倍,第一抗体的孵育时间为60 min,电化学检测环境的pH值为7.4时,BPA浓度在1~250 ng/mL范围内与还原峰电流值呈线性关系,其相关系数(r2)为0.993,检出限为0.85 ng/mL。该方法具有良好的特异性、重现性、稳定性,加标回收率为98.3%~104%,说明BPA免疫传感器对实际水样中双酚A的检测具有潜在的应用价值。
中文关键词:纳米金  聚赖氨酸  丝网印刷电极(SPCE)  免疫传感器  双酚A(BPA)
 
Sensitive Detection of Bisphenol A Using an Immunosensor Based on Nano-gold/Poly-L-lysine Modified Screen-printed Electrode
Abstract:In this paper,an electrochemical immunosensor for the detection of bisphenol A(BPA) was constructed,based on the preparation of a poly-L-lysine/nano-gold modified electrode by electrodeposition,with disposable screen printed carbon electrode as the substrate.Results showed that,when the dilution factors for the antigen and the primary antibody were 1 000 times,the incubation time for the primary antibody was 60 min,and the pH value for the electrochemical detection environment was 7.4,there existed a good linear relationship between reduction peak current and BPA in the concentration range of 1-250 ng/mL,with a correlation coefficient(r2) of 0.993 and a detection limit of 0.85 ng/mL.With good specificity,reproducibility and stability,this method was applied in the detection of BPA in samples with spiked recoveries of 983%-104%,indicating that the BPA immunosensor has an application potential in the detection of bisphenol A in actual water samples.
Key Words:nano-gold  poly-L-lysine  screen printed carbon electrode(SPCE)  immunosensor  bisphenol A(BPA)
引用本文:何祖宇,李普旺,周闯,王超,吕明哲,宋书会,刘运浩,杨子明.基于纳米金/聚赖氨酸修饰的丝网印刷电极免疫传感器对双酚A的灵敏检测[J].分析测试学报,2020,39(12):1508-1514.
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