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纳米银刻蚀比色法检测褪黑激素
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作者单位
肖传豪 1.濮阳职业技术学院
2.河南大学濮阳工学院 
基金项目:国家自然科学基金资助项目(21801215)
中文摘要:该文构建了一种基于氯金酸刻蚀球形纳米银检测褪黑激素的简单、高灵敏比色探针。纳米银可被氯金酸氧化刻蚀为Ag+,同时还原生成的纳米金沉积在刻蚀后的纳米银表面,导致其溶液的吸光度降低和颜色增强(由黄色变为橘黄色)。当向体系中加入褪黑激素时,氯金酸被还原,抑制了纳米银的刻蚀,从而使得溶液吸光度增加和颜色变浅。结果显示,在0.1 nmol/L~1.0 mmol/L范围内,褪黑激素浓度对数值(lgC)与其吸光度改变值(ΔA)呈良好的线性关系,线性方程为ΔA=0.049 8+0.516lgC,相关系数(R2)为0.996 4,检出限为0.09 nmol/L。该方法成功应用于人体尿液和葡萄中的褪黑激素的测定。
中文关键词:球形纳米银  比色  刻蚀  褪黑激素
 
Colorimetric Detection of Melatonin Based on Silver Nanoparticles Etching
Abstract:In this paper,a simple and highly sensitive colorimetric probe was constructed for the determination of melatonin based on the etching of spherical Ag nanoparticles(AgNPs) by HAuCl4.The AgNPs could be oxidized and etched into Ag+ by HAuCl4,and the reduced Au was deposited on the surface of the etched AgNPs,resulting in the decrease of absorbance and the variation of solution color from yellow to orange.However,in the presence of melatonin,HAuCl4 was reduced by melatonin,inhibiting the etching of AgNPs,and resulting in an increase in absorbance and a decrease in color.Results showed that there existed a good linear relationship between the change values of absorbance(ΔA) and the logarithm values of melatonin concentration(lgC) in the range of 0.1 nmol/L-1.0 mmol/L,with a linear equation of ΔA=0.049 8+0.516lgC,a correlation coefficient(R2) of 0.996 4 and a detection limit of 0.09 nmol/L.This method was successfully applied to the determination of melatonin in human urine and grape with satisfactory results.
Key Words:spherical Ag nanoparticles  colorimetry  oxidation etching  melatonin
引用本文:肖传豪.纳米银刻蚀比色法检测褪黑激素[J].分析测试学报,2020,39(12):1544-1547.
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