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银莱汤中主要成分的UPLC多波长宽动态测定及质量评价研究
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郁映婷,刘洁,白辰,赵淑军,杨洁,吴浩,潘绮雪,刘铁钢,肖红斌 1.北京中医药大学中药学院2.北京中医药大学中医药研究院3.北京中医药大学中药分析与转化研究中心4.北京中医药大学中医学院5.新疆石河子大学药学院 
基金项目:北京市科技新星项目(Z181100006218083);北京中医药大学研究生创新创业课题(2019-JYB-XSCXCY-56)
中文摘要:采用超高效液相色谱(UPLC)定量测定银莱汤中10种有效成分的含量并用于其质量评价。采用Waters ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8 μm),以0.1%甲酸水溶液-0.1%甲酸乙腈为流动相梯度洗脱,3波长切换(0~10 min,320 nm;10~16 min,350 nm;16~30 min,320 nm)测定。结果表明,该方法可在30 min内实现银莱汤中10种有效成分的基线分离,10种成分在各自质量浓度范围内线性关系良好(r≥0.999 2),检出限为0.018 8~0.075 0 μg·mL-1,定量下限为0.062 5~0.250 μg·mL-1。检测得到银莱汤中10种成分的平均含量为0.204~38.129 mg·g-1,其中黄芩苷的含量最高,槲皮苷的含量最低。将该方法用于3批样品分析,所测成分含量稳定、一致性好。该文建立的分析指标相对全面、合理可行,能更好地表征及评价银莱汤质量。
中文关键词:银莱汤  超高效液相色谱  波长切换  含量测定  质量评价
 
Study on Dynamic Determination of Main Components in Yinlai Decoction by UPLC with Multi-wavelength and Their Quality Evaluation
Abstract:A method of ultra high performance liquid chromatography(UPLC) was developed for the quantitative determination of 10 active components in Yinlai decoction,which was subsequently used for the quality evaluation of Yinlai decoction.The chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column(2.1 mm×100 mm,1.8 μm) by gradient elution with 0.1% formic acid water and 0.1% formic acid acetonitrile as mobile phases.The detection wavelength was firstly set at 320 nm for neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,sinapine thiocyanate,forsythoside I in the first 10 min,then switched to 350 nm for forsythoside A,quercitrin from 10 to 16 min,and finally switched to 320 nm for baicalin,oroxylin A-7-O-β-D-glucuronide,wogonoside from 16 to 30 min.Results showed that the method completed the baseline separation for 10 active components in Yinlai decoction within 30 min.There were good linear relationships for 10 components in the certain concentration ranges with their correlation coefficients(r) not less than 0.999 2.The limits of detection and limits of quantitation were in the ranges of 0.018 8-0.075 0 μg·mL-1 and 0.062 5-0.250 μg·mL-1,respectively.The content range for 10 components ranged from 0.204 to 38.129 mg·g-1,in which baicalin was the highest,while quercitrin was the lowest.The method was used in the detection of three batches of sample,and it was found that the contents of the measured components were stable and consistent.The established analytical indexes are relatively comprehensive,reasonable and feasible,and could be used for better characterization and evaluation of the quality of Yinlai decoction.
Key Words:Yinlai decoction  ultra high performance liquid chromatography  wavelengths switching  quantitative analysis  quality evaluation
引用本文:郁映婷,刘洁,白辰,赵淑军,杨洁,吴浩,潘绮雪,刘铁钢,肖红斌.银莱汤中主要成分的UPLC多波长宽动态测定及质量评价研究[J].分析测试学报,2021,40(1):99-105.
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