| 基于代谢组学技术的快速蒸发电离质谱与智能手术刀联用实时鉴别果汁掺伪方法研究(英文) |
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| 基金项目:the National Key Research and Development Program of China(2018YFC1604306)；the Open Fund of Henan Key Laboratory of Tea Comprehensive Utilization in South Henan(HNKLTOF2019002) |
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| 中文摘要:近年来，果汁掺伪问题日益突出。作为一种新型的实时鉴别方法，智能手术刀(IKnife)与快速蒸发电离质谱(REIMS)联用技术无需样品前处理即可解决这一问题。该文采用代谢组学指纹识别技术建立橙汁、苹果汁和葡萄汁的实时鉴别方法，并对其掺假水平进行预测。主成分分析-线性判别分析联用法建立的判别模型能够区分不同类型的果汁，排除20%交叉验证法的正确率为9728%，未知样品实时鉴别正确率高达100%。筛选出不同果汁中的标志物，并对部分化合物进行鉴定。结果表明，此方法能够成功区分掺有10%-50%苹果汁和葡萄汁的橙汁。通过对比分析REIMS单极质谱和串联质谱(REIMS/MS)的检测结果，两种方法建立的偏最小二乘分析模型均能准确预测掺假水平(所有模型的R2和Q2均大于082)。与传统的REIMS方法相比，REIMS/MS的预测值更加精确。 |
| 中文关键词:智能手术刀 快速蒸发电离质谱(REIMS) 代谢组学 果汁 掺假 快速蒸发电离串联质谱(REIMS/MS) |
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| Real-time Assessment of Fruit Juices Adulteration by Rapid Evaporative Ionization Mass Spectrometry with IKnife Based on Metabolomics |
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| Abstract:In recent years,fruit juice adulteration has become an increasingly important issue.Rapid evaporative ionization tandem mass spectrometry(REIMS),as a novel method for real time discrimination of samples,could solve this problem without any sample preparation.In this paper,a metabolomics fingerprinting approach for real time authentication of orange,apple and grapefruit juices was developed.A discriminate model established by principal component analysis(PCA) and linear discriminate analysis(LDA) was able to distinguish different types of fruit juice with a correct classification of 9728% in leave 20% out cross validation and a 100% burns correctly identified in real time recognition.Characteristic m/z markers were detected and some of them were identified.Adulterated orange juices with apple and grape juices at different percentages could be well distinguished from the level of 50% down to 10%.Furthermore,the adulterated samples were analyzed by optimized single MS and tandem MS/MS REIMS(REIMS and REIMS/MS).Partial least squares analysis was successfully used to predict the level of the adulterants(both R2 and Q2>082 for all models).REIMS/MS could offer higher prediction accuracy compared with the conventional REIMS. |
| Key Words:iKnife rapid evaporative ionization mass spectrometry(REIMS) metabolomics fruit juice adulteration rapid evaporative ionization tandem mass spectrometry(REIMS/MS) |
| 引用本文：王慧君，文汶，曹学丽，韩天.基于代谢组学技术的快速蒸发电离质谱与智能手术刀联用实时鉴别果汁掺伪方法研究(英文)[J].分析测试学报,2021,40(2):247-255. |
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