| 固相基底电喷雾电离质谱分析固状与粘稠状药物的基质效应(英文) |
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| 基金项目:Project on the National Natural Science Foundation of China(21804053,41827804) |
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| 中文摘要:固状与粘稠状辅剂常用于制造颗粒、片剂、胶囊或膏状药品，并能有效地保持药效成分活性的稳定性。基于固相基底的敞开式电喷雾电离质谱技术已被发展用于药品的直接分析。然而，固状与粘稠状基质对药物分子直接质谱检测的影响规律还不明确。该研究构建了不同黏度和不同溶解度的基质体系，采用木签(牙签)为代表的固相基底电喷雾电离质谱考察了几种模式药物分子在固状与粘稠状基质中的质谱响应规律。结果发现，易溶解的基质电离产生显著的离子干扰和抑制效应，而难溶的基质尽管不产生离子干扰，但在溶剂作用下可变成粘稠状从而抑制药物的离子化。进一步研究表明，药物分子的质谱信号随着基质的黏度增加(1~500 cP)呈幂函数降低(r2>0.95)。该研究将增进理解固体与粘稠样品直接质谱分析的基质效应与信号响应规律。 |
| 中文关键词:基质效应 固状药物 粘稠药物 固相基底电喷雾电离 敞开式质谱 |
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| Matrix Effect in Direct Analysis of Raw Solid and Viscous Drugs by Solid-substrate Electrospray Ionization Mass Spectrometry |
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| Abstract:Solid and viscous auxiliaries are often used for manufacturing granules,tablets,capsules and ointments in pharmaceutical industry,which emerge in the promising forms to maintain the stability and activity of various drugs by a variety of matrices.Ambient mass spectrometry(MS) with wooden-tip electrospray ionization(ESI) has been successfully developed for direct analysis of raw drug samples.However,matrix effect in such an ambient ESI-MS analysis on solid and viscous drugs has not yet been well understood.In this work,ionization responses to several modes of drugs in various viscous and solid matrices were investigated using a typical wooden-tip ESI-MS.Ionization responses were found to be related to the solubility of solid matrices.Ionic suppression and interference were observed in soluble solid matrices,while significant ionic interference was not generated in insoluble matrices.However,by adding spray solvent,insoluble matrices were changed in a viscous state suppressing spray ionization process.Further investigation revealed that ionization responses to analytes were decreased as a power function(r2>0.95) with the increase of matrix viscosity from 1 cP to 500 cP in this study.Therefore,this study may provide a good understanding for matrix effect and signal response in substrate ESI-MS analysis on solid and viscous samples. |
| Key Words:matrix effect solid samples viscous samples solid substrate electrospray ionization ambient mass spectrometry |
| 引用本文：狄丹丹，袁子程，伍琳，姚亚男，胡斌.固相基底电喷雾电离质谱分析固状与粘稠状药物的基质效应(英文)[J].分析测试学报,2021,40(2):270-275. |
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