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实时直接分析-四极杆飞行时间质谱法快速鉴别油茶籽油真伪
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作者单位
胡谦,张九凯,邢冉冉,陈颖 1.中国检验检疫科学研究院2.北京工商大学食品与健康学院 
基金项目:“十三五”国家重点研发计划项目(2017YFF0211301,2018YFC1603504)
中文摘要:建立了实时直接分析-四极杆飞行时间质谱(DART-QTOF-MS)技术结合化学计量学分析快速鉴别油茶籽油的方法。4种食用油(油茶籽油、菜籽油、花生油和大豆油)用甲醇-异丙醇(50∶50,含5 mmol/L乙酸铵)稀释后经实时直接分析离子源解吸电离,在正离子模式下以TOF MS和信息依赖采集模式获得质谱数据,对4种食用油的质谱图进行了初步鉴定,并采用化学计量学建立主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)模型,以实现数据可视化和油茶籽油的快速鉴别。结果表明,4种食用油的质谱图存在明显差异,主要为甘油三酯(TAG)氨加合离子[TAG+NH4]+(m/z 850~1 100)、甘油二酯(DAG)氨加合离子[DAG+NH4]+(m/z 600~700)和DART源内碎片离子,共检出20种甘油三酯和6种甘油二酯。进一步采用化学计量学对4种植物油指纹图谱进行分析,发现该方法可准确鉴别和预测油茶籽油、菜籽油、花生油和大豆油,具有前处理简单、分析速度快、操作方便和准确可靠等优点,可用于油茶籽油的鉴别研究。
中文关键词:实时直接分析  油茶籽油  真伪  化学计量学
 
Fast Identification on Authenticity of Camellia Oil by Direct Analysis in Real Time-Quadrupole Time-of-flight Mass Spectrometry
Abstract:A rapid identification method for camellia oil was established by direct analysis in real time-quadrupole time-of-flight mass spectrometry(DART-QTOF-MS) combined with chemometrics analysis.Camellia oil,rapeseed oil,peanut oil and soybean oil were accurately weighed,respectively.After dilution,each of them were executed a desorption ionization by DART.In the positive ion mode,the MS data were obtained by TOF MS and information dependent acquisition(IDA) mode,and the mass spectra of four edible oils were preliminarily identified.Meanwhile,the models of principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) were established by chemometrics to realize the data visualization and the rapid identification and prediction of camellia oil.Results showed that the main contents in the mass spectra were triacylglycerol ammoniated ions [TAG+NH4]+(m/z 850-1 100),diacylglycerol ammoniated ions [DAG+NH4]+(m/z 600-700) and DART source debris ions.There were obvious differences in the mass spectra of the four edible oils.A total of 20 triacylglycerols and 6 diacylglycerols were detected.The chemometrics analysis could be used for accurate identification and prediction of camellia oil,rapeseed oil,peanut oil and soybean oil.This method is feasible for the rapid identification on authenticity of camellia oil with its advantages of simple pretreatment,quick analysis and easy operation.
Key Words:direct analysis in real time  camellia oil  authenticity  chemometrics
引用本文:胡谦,张九凯,邢冉冉,陈颖.实时直接分析-四极杆飞行时间质谱法快速鉴别油茶籽油真伪[J].分析测试学报,2021,40(2):282-287.
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