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SC-ICP-MS法测定金属元素的方法研究
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作者单位
傅霜,赵珍阳,龚子珊,王世功,田中朝,孙传强,赵玉新,张勇,汪曣 1.天津大学精密仪器与光电子工程学院天津3000272.山东东仪光电仪器有限公司山东烟台264000 
基金项目:山东省重大科技创新工程项目(2018YFJH0302)
中文摘要:为了使用单细胞电感耦合等离子体质谱(SC-ICP-MS)方法准确测定单个细胞中的铬(Cr)、锰(Mn)、铁(Fe)、铜(Cu)和锌(Zn)等多种内源性金属元素,该文基于动态反应池(DRC)模式对目标分析物的反应气流量和极杆抑制参数q(RPq)进行了优化,并研究了进样速度、细胞密度、驻留时间等因素对SC-ICP-MS检测的影响。分别采用细胞悬液直接进样、使用超声波探头使细胞悬液中的细胞破碎后进样和使用浓硝酸消解细胞后进样的ICP-MS测定结果对SC-ICP-MS定量结果的准确性进行验证分析。实验结果表明,可采用超声波破碎细胞的ICP-MS测定结果评估SC-ICP-MS测定的单细胞内Zn和Cu含量的准确性,采用酸消解细胞的ICP-MS检测结果验证单细胞内Fe和Cr的含量。缺少细胞标准物质时对SC-ICP-MS方法定量结果进行多角度验证是必要的。研究表明,使用SC-ICP-MS法可以较好地进行单细胞元素相关分析。
中文关键词:单细胞电感耦合等离子体质谱(SC-ICP-MS)  方法验证  单细胞分析  微量元素  动态反应池(DRC)技术
 
Method Study of Single Cell Inductively Coupled Plasma Mass Spectrometry for Determination of Metal Elements
Abstract:A single cell inductively coupled plasma mass spectrometry(SC-ICP-MS) was adopted to determine the intrinsic metal elements such as chromium(Cr),manganese(Mn),iron(Fe),copper(Cu) and zinc(Zn) in an individual cell in this article.Meanwhile,the reaction gas flow rate and the quadrupole rejection parameter q(RPq) of the analytes in dynamic reaction cell(DRC) mode were optimized for accurate determination.Besides,the effects of sample flow rate,cell density and dwell time on the detection of SC-ICP-MS were investigated.The accuracy for the quantitative results of SC-ICP-MS was validated by using the results of ICP-MS determination after cell resuspension,cell lysis by ultrasonic probe and cell digestion with concentrated nitric acid.Experimental results showed that the accuracy of quantitative Zn and Cu in single cell determined by SC-ICP-MS could be evaluated by the results of ICP-MS determination after cell lysis by ultrasound. The determined Fe and Cr in single cells could be verified by the results of cell acid digestion ICP-MS. In the absence of cell reference materials, a multi-angle validation for the quantitative results of SC-ICP-MS is necessary. Studies have shown that SC-ICP-MS could be better used for the analysis of related elements in single cell.
Key Words:single cell inductively coupled plasma mass spectrometry(SC-ICP-MS)  method validation  single cell analysis  trace elements  dynamic reaction cell(DRC)
引用本文:傅霜,赵珍阳,龚子珊,王世功,田中朝,孙传强,赵玉新,张勇,汪曣.SC-ICP-MS法测定金属元素的方法研究[J].分析测试学报,2021,40(3):389-395.
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