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高效液相色谱-分段检测法同时测定烟草中14种多酚类化合物
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欧阳璐斯,赖燕华,王予,陶红,黄翼飞 广东中烟工业有限责任公司技术中心广东广州510385 
基金项目:广东中烟工业有限责任公司资助项目(粤烟工[2017]科字第9号)
中文摘要:利用高效液相色谱-二极管阵列检测器(HPLC-DAD),建立了同时分析烟草中新绿原酸、绿原酸、隐绿原酸、东莨菪苷、咖啡酸、七叶亭、对香豆酸、阿魏酸、莨菪亭、7 羟基香豆素、芦丁、槲皮苷、山奈酚 3 O 芸香糖苷和槲皮素14种多酚化合物的方法。称取002 g烟末,加入5 mL 50%甲醇,超声萃取30 min,取上清液过水相滤膜后,采用Agilent SB-C18柱(30 mm×150 mm,18 μm)进行分离,以005 mol/L磷酸二氢钾水溶液和甲醇为流动相,采用分段式检测,获得14种多酚类化合物在各自最佳检测波长下的信号,实现了良好分离。结果表明,14种多酚类化合物的色谱峰面积与其质量浓度呈良好的线性关系,相关系数r2>0999 7,检出限为001~015 μg/mL,定量下限为003~050 μg/mL,日内相对标准偏差(RSD)为070%~36%,日间RSD为40%~64%,加标回收率为952%~109%。该方法具有良好的灵敏度、精密度和回收率,适合于烟草样品中多种多酚类化合物的同时测定。
中文关键词:高效液相色谱  二极管阵列检测器  分段检测  烟草  多酚
 
Simultaneous Determination of Fourteen Polyphenols in Tobacco by Piecewise Detection Based on Liquid Chromatography
Abstract:A high performance liquid chromatography with diode array detector(HPLC-DAD) was developed for the simultaneous determination of 14 polyphenols,including neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,scopolin,caffeic acid,esculetin,p coumaric acid,ferruric acid,scopoletin,7 hydroxycoumarin,rutin,quercitrin,kaempferol 3 O β rutinoside and quercetin.002 g tobacco powder sample was ultrasonically extracted with 5 mL of 50%methanol for 30 min,and then the supernatant was put to pass through an aqueous phase filtration membrane.14 polyphenols were separated at their optimal detection wavelengths on an Agilent SB-C18 column(30 mm×150 mm,18 μm),with 005 mol/L potassium dihydrogen phosphate aqueous solution and methanol as mobile phases.There existed good linear relationships between peak areas and mass concentrations of the analytes,with their correlation coefficients(r2) larger than 0999 7.The limits of detection and limits of quantitation were in the ranges of 001-015 μg/mL and 003-050 μg/mL,while the intra day and inter day relative standard deviations(RSD) were 070%-36%and 40%-64%,respectively.The spiked recoveries ranged from 952%to 109%.The proposed method could be applied to the simultaneous determination of a variety of polyphenols in tobacco and cigarettes with good sensitivity,precision and recovery.
Key Words:high performance liquid chromatography  diode array detector  piecewise detection  tobacco  polyphenols
引用本文:欧阳璐斯,赖燕华,王予,陶红,黄翼飞.高效液相色谱-分段检测法同时测定烟草中14种多酚类化合物[J].分析测试学报,2021,40(3):411-416.
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