| 纳米脂质体放大血糖仪信号用于蓖麻毒素的即时检测（英文） |
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| 基金项目:The National Key Research and Development Program of China(2018YFC1602600);the National Natural Science Foundation of China(21974109);the Fundamental Research Funds for the Central Universities(XDJK2019TY003);the Fund of Chongqing Research Program of Basic Research and Frontier Technology(cstc2017jcyjAX0091) |
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| 中文摘要:蓖麻毒素是已知的最致命的毒素之一，可作为一种生物武器并造成潜在的环境和食品污染。常用的蓖麻毒素检测方法存在耗时长、需要复杂且专业的实验室操作等缺点。为了克服该缺点，实现简单、快速、灵敏的蓖麻毒素非实验室检测，该文提出了一种利用负载有淀粉葡萄糖苷酶的纳米脂质体(ALN)放大血糖仪信号实现蓖麻毒素B链(RTB)即时检测的新方法。首先，将磁珠上的核酸适配体与ALN上的Blocker链杂交，构建一种集靶物识别、磁分离和信号放大功能于一体的探针。当加入RTB后，RTB与核酸适配体特异性结合，使Blocker连接的ALN从磁珠表面释放出来。磁分离后，用Triton X-100裂解上清液中的ALN，释放出纳米脂质体中包裹的淀粉葡萄糖苷酶，从而水解淀粉产生大量葡萄糖。最后，利用血糖仪可在10 s内读取葡萄糖的量。因此，可通过血糖仪响应值的增加来检测RTB。该方法的线性范围为03~30 μg/mL，检出限为 67 ng/mL。该方法显著提高了检测的灵敏度和选择性，有望用于其他靶物的即时检测。 |
| 中文关键词:即时检测 纳米脂质体 蓖麻毒素B链(RTB) 血糖仪(PGM) |
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| Scientific PapersA Novel Nanoliposome Signal Amplification Platform for Sensitive Personal Glucose Meter Based Point-of-Care Detection of Ricin |
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| Abstract:Ricin is one of the most deadly toxins known,which could be used as a biological weapon causing potential environmental and food pollution.However,common methods for ricin detection are time consuming and limited due to their complicate and professional laboratory operations.To overcome these limitations of the common detection methods and achieve a simple,rapid and sensitive non laboratory detection of ricin,herein,a novel method was presented for the point of care(POC) detection of ricin B chain(RTB) by using amyloglucosidase loaded nanoliposome(ALN) to amplify the personal glucose meter(PGM) signal.Initially,a hybrid probe was constructed by hybridizing an aptamer on magnetic beads(MBs) with a blocker on ALN,combining the functions of target recognition,magnetic separation and signal amplification together.Then,in the presence of RTB,the blocker linked ALN was released from the surface of MBs after magnetic separation with the formation of RTB aptamer complex.Subsequently,as obtained ALN supernatant was lysed with Triton X-100 to liberate amyloglucosidase to hydrolyze amylose,generating a great number of glucose.Finally,the glucose was quantitatively read out by the PGM in 10 s.Therefore,RTB can be detected by using the enhancement of PGM value.The linear range for the detection was from 0.3 μg/mL to 30 μg/mL and the limit of detection(LOD) was 67 ng/mL.Results showed that the developed method remarkably improved the sensitivity and selectivity for RTB detection,and exhibited a great potential in POC detection of other targets. |
| Key Words:point of care testing nanoliposome ricin B-chain(RTB) personal glucose meter(PGM) |
| 引用本文：门晨,李原芳,黄承志,甄淑君.纳米脂质体放大血糖仪信号用于蓖麻毒素的即时检测（英文）[J].分析测试学报,2021,40(4):534-542. |
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