•  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
期刊检索


检索
检索项:
检索词:
总目录
  • WCSB9︱2019(第九届)世界采样和混样大会 查看全文>>
  • 关于召开第十五届全国青年分析测试学术报告会的通知 查看全文>>
友情链接
薏苡仁中黄曲霉毒素G1的动态表面增强拉曼光谱检测
    点此下载全文
作者单位
任菲,于治国,陆峰 1.沈阳药科大学药学院辽宁沈阳110016
2.第二军医大学药学院上海200082 
基金项目:国家重点研发计划(2019YFC0312603);国家科技重大专项(2018ZX09J18112);生物安全重点专项(19SWAQ20)
中文摘要:中药具有不良反应少,疗效独特等优点,越来越受到国内外的关注。但中药在生产、采集、加工、运输、贮藏等方面极易发生霉变而污染黄曲霉毒素。黄曲霉毒素具有很强的毒性,不仅影响中药质量和疗效还会对人体肝脏等器官造成极大损害。该文建立了中药材薏苡仁中黄曲霉毒素G1(Aflatoxin G1,AFG1)的动态表面增强拉曼光谱(Dynamic Surface-enhanced Raman Spectroscopy,D-SERS)的快速分析方法。采用简单擦拭法提取薏苡仁表面的黄曲霉毒素G1,因此SERS图谱中干扰峰较少。并且以金纳米颗粒为增强基底,优化了检测条件,进行了黄曲霉毒素G1的D-SERS的检测与分析。结果表明,方法的线性范围为8~320 μg/kg,线性方程为y=0.029 3x+1.730 7,相关系数(r2)为0.988 4。检出限(LOD)为5.5 μg/kg,相对标准偏差(RSD)为11%。该方法的线性良好,灵敏度和重现性均能满足薏苡仁中黄曲霉毒素G1的快速检测要求,且操作简单、快速、节约成本,对中药材中黄曲霉毒素G1的现场实时快速分析具有良好的应用潜力。
中文关键词:擦拭法  黄曲霉毒素G1  薏苡仁  动态表面增强拉曼光谱
 
Detection of Aflatoxin G1 in Coix Seed by Dynamic Surface-enhanced Raman Spectroscopy
Abstract:Traditional Chinese medicines(TCMs) play a significant role due to their less adverse reactions and unique medical efficiency,which have attracted more and more attention both at home and abroad.However,TCMs are prone to mildew and generate aflatoxins in production,collection,processing,transportation and storage.Aflatoxins with strong toxicity could not only affect the quality and efficacy of traditional Chinese medicines,but also cause great damage to the liver and other organs of human.A rapid dynamic surface enhanced Raman spectroscopic(D-SERS) method was established for the detection of aflatoxin G1(AFG1) in coix seed,a kind of traditional Chinese medicine.The aflatoxin G1 on the surface of coix seed was extracted by wiping method,while not all of the ingredients in coix seed could be extracted.Therefore,there was less interference of coix seed in SERS spectrum.Aflatoxin G1 was analyzed by D-SERS with gold nanoparticles as the enhanced substrate,which are characterized by their good stability.The detection conditions were optimized.The calibration curves were linear in the range of 8-320 μg/kg,with a linear formula as y=0.029 3x+1.730 7,and a correlation coefficient(r2) of 0.988 4.The limit of detection(LOD) was 5.5 μg/kg,and the relative standard deviation(RSD) was 11%.Results showed that the established method was simple,rapid and cost saving,and it could meet the requirements for rapid detection of aflatoxin G1 in coix seed with good linearity,sensitivity and reproducibility.Therefore,this method has a good application potential in real time and rapid analysis of aflatoxin G1 in Chinese herbal medicines.
Key Words:wiping method  aflatoxin G1  coix seed  dynamic surface enhanced Raman spectroscopy
引用本文:任菲,于治国,陆峰.薏苡仁中黄曲霉毒素G1的动态表面增强拉曼光谱检测[J].分析测试学报,2021,40(4):612-616.
摘要点击次数: 1532
全文下载次数: 663
查看全文  下载PDF阅读器