基于非标记核酸适配体荧光法检测灌溉水中汞离子的研究 |
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基金项目:北京市农林科学院院青年科研基金项目(QNJJ201903) |
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中文摘要:该文利用汞特异性核酸适配体和可以嵌入双螺旋脱氧核糖核酸中的荧光染料 PicoGreen 建立了一种 快速检测汞离子的荧光分析方法。通过荧光信号强度来测定汞离子浓度,并获得了汞离子浓度的对数和荧光 强度之间的线性关系。方法的线性范围为 0. 001~100 μg/mL,检出限达到 0. 38 ng/mL。在 As3+ 、Pb2+ 、Cd2+ 、 Cr2+ 、K+ 等干扰离子存在的情况下,具有很好的特异性。同时,在灌溉水实际样品检测中的回收率为95. 2%~ 97. 9%,相对标准偏差为2. 3%~4. 0%。该方法利用核酸适配体和荧光染料实现了对汞离子的痕量检测,是一 种简单、快速、灵敏的测定方法。 |
中文关键词:核酸适配体 汞 快速检测 荧光法 PicoGreen |
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Detection of Mercury by a Label-free Aptamer-based Fluorescent Assay |
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Abstract:In this paper,a rapid fluorescence assay for the detection of mercury ions was developed using mercury-specific aptamer and PicoGreen,a fluorescent dye embedded in double helix DNA. Mercury ion concentration was determined by fluorescence signal intensity,and the linear relation? ship between the logarithm of mercury ion concentration and fluorescence intensity was obtained. The linear range of the method was 0. 001-100 μg/mL,and the detection limit was 0. 38 ng/mL. In the presence of interference ions such as As3+ ,Pb2+ ,Cd2+ ,Cr2+ ,K+ ,it has a good specificity. Mean? while,the irrigation water samples with different concentrations of Hg2+ showed a good recovery of 95. 2%-97. 9% and the relative standard deviation of 2. 3%-4. 0%. The method is simple,rapid and sensitive,and is applicable for the determination of mercury ions by using aptamer and fluores? cent dye. |
Key Words:aptamer mercury rapid detection fluorescent assay PicoGreen |
引用本文:王 楠,陈佳祎,陆安祥,栾云霞,卢静华.基于非标记核酸适配体荧光法检测灌溉水中汞离子的研究[J].分析测试学报,2021,40(8):1203-1207. |
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