| 一种基于 1，4-亲核加成反应的新型荧光探针及其在食品和活细胞中的应用 |
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| 基金项目:国家自然科学基金项目（21907026）；湖南省自然科学基金资助项目（2019JJ50009）；湖南省教育厅资助项目（18B369， 20A338）；衡阳师范学院学科群建设项目（18XKQ07）；衡阳师范学院金属有机功能材料重点实验室（GN18K03）；常德市指导性科技计划项目（2020ZD28）；湖南文理学院科学研究项目（20YB23） |
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| 中文摘要:该文构建了一种简单、有效的亚硫酸盐荧光探针（CQ）。该探针以N-甲基喹啉三氟甲磺酸盐片段为识别基团，通过乙烯桥键与4-氰基苯乙腈片段连接。在与亚硫酸盐作用后，探针CQ表现出明显的荧光增强，同时，探针溶液颜色由无色变成黄色，该过程可被肉眼直接观察。通过核磁共振氢谱，详细研究了探针CQ与亚硫酸盐的反应机理。该探针对亚硫酸盐具有较快的荧光响应（20 s），适于亚硫酸盐的实时检测。在PBS缓冲溶液（DMF∶H2O = 1∶9，体积比，pH 7.4）中，探针CQ的荧光强度与亚硫酸盐的浓度在0～10 μmol/L范围内呈现良好的线性关系，检出限低至25 nmol/L。选择性实验结果表明，探针CQ对亚硫酸盐具有较高的选择性，可以满足实际应用的要求。重要的是，该探针可用于糖、酒等真实样品和活细胞中亚硫酸盐的检测，为食品中亚硫酸盐的监测及生物体内亚硫酸盐的成像提供了强有力的分析方法。 |
| 中文关键词:亚硫酸盐 荧光探针 食品 细胞成像 |
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| Construction of a New Fluorescent Probe Based on 1，4-Nucleophilic Addition Reaction for Sulfite and Its Application in Food and Living Cells |
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| Abstract:In this study， a simple but effective fluorescent probe（CQ） was constructed for the detection of sulfite. The N-methylquinoline trifluoromethanesulfonate fragment as the sulfite response site was linked to the 4-cyanobenzoacetonitrile fragment using a C? C bond. Upon treatment with sulfite， the probe CQ showed an obvious fluorescence enhancement. Meanwhile， the color of the CQ solution changed from colorless to yellow， which could be directly observed with naked eye. The reaction mechanism of probe CQ towards sulfite was studied in detail by 1H NMR spectra. Notably， the fluorescence response of the probe to sulfite was very fast（20 s）， which was suitable for the real-time detection of sulfite. In PBS buffer solution（DMF∶H2O=1∶9， volume ratio， pH 7.4）， there existed a good linear relationship between the fluorescence intensity at 511 nm and concentration of sulfite in the range of 0－10 μmol/L， with a detection limit of 25 nmol/L. The selectivity experiments indicated that probe CQ has a high selectivity for sulfite， which could satisfy the requirements for practical application. More importantly， the probe could not only be applied to the detection of sulfite in real food samples， but also realize the imaging of sulfite living cells， which provides a powerful analytical method for the monitoring of sulfite in food and biological systems. |
| Key Words:sulfite fluorescent probe food cell imaging |
| 引用本文：张春香，唐斯萍，李雯倩，申有名，谷 标.一种基于 1，4-亲核加成反应的新型荧光探针及其在食品和活细胞中的应用[J].分析测试学报,2021,40(9):1286-1292. |
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