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高效液相色谱-蒸发光散射法测定液态乳中乳果糖含量
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作者单位
孙 佩,任 硕,陈柔含,古淑青,钮 冰,邓晓军 1. 上海大学 生命科学学院 食品工程系上海 2004362. 上海海关动植物与食品检验检疫技术中心 上海 2001353. 上海市农产品质量安全中心上海 201708 
基金项目:上海市科委农业领域项目(19391901500);上海专业技术服务平台项目(20DZ2291900);长三角科技合作项目(19395810100,1804b06020349)
中文摘要:液态乳中乳糖和乳果糖是同分异构体,因保留特性相似,成为色谱法分离检测乳果糖的难点。目前,虽有多种液相色谱检测乳果糖的报道,但方法在前处理的简便性、色谱柱的适用性及分离效果的稳定性等方面存在不足。该研究建立了高效液相色谱-蒸发光散射(HPLC-ELSD)检测液态乳中乳果糖的方法。采用0.4 mol/L醋酸铵(pH 3.8)沉降蛋白,氨基色谱柱分离,蒸发光散射检测器检测。液态乳中乳果糖与乳糖得到了良好的分离,在1.0~100 mg/L范围内线性关系良好(r2 = 0.998 0),检出限为15 mg/L,定量下限为50 mg/L,回收率为85.1%~110%,相对标准偏差(RSD)不大于7.5%。该方法操作简单,可在8 min内完成检测,结果重复性好、准确度高,满足液态乳中乳果糖的日常检测要求。
中文关键词:乳果糖  高效液相色谱  蒸发光散射  液态乳
 
Quantitative Determination of Lactulose in Liquid Milk by High Performance Liquid Chromatography with Evaporative Light Scattering Detection
Abstract:Lactose and lactulose in liquid milk are isomers with similar retention properties, which makes separation and detection on lactulose by chromatography difficult. At present, although a variety of liquid chromatography detection methods have been reported, there are some problems in the simplicity of the pretreatment, the applicability of the chromatographic column and the stability of the separation effect. A method for the determination of lactulose in liquid milk by high performance liquid chromatography with evaporative light scattering detection(HPLC-ELSD)was developed. The protein was deposited with 0.4 mol/L ammonium acetate(pH 3.8). The sugar analysis column was used for separation, and the evaporative light scattering detector was applied to detection. There was a good linear relationship for lactulose in liquid milk in the range of 1.0-100 mg/L, with a correlation coefficient(r2) of 0.998 0. The detection limit and the quantitative limit were 15 mg/L and 50 mg/L, respectively. The recoveries were in the range of 85.1%-110%, with relative standard deviations(RSDs) not more than 7.5%. The method is simple to operate, and could be completed within 8 min. It could meet the requirements for daily detection of lactulose with good repeatability and high accuracy.
Key Words:lactulose  high performance liquid chromatography  evaporative light scattering  liquid milk
引用本文:孙 佩,任 硕,陈柔含,古淑青,钮 冰,邓晓军.高效液相色谱-蒸发光散射法测定液态乳中乳果糖含量[J].分析测试学报,2021,40(9):1391-1395.
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