| 高效液相色谱法测定食品接触材料及制品中9种酚类物质的迁移量 |
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| 基金项目:江苏省自然科学基金青年项目（BK20201109）；江苏理工学院产学研项目（KYH20072）；南京海关科研计划项目（2020KJ26） |
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| 中文摘要:该文建立了苯酚、甲酚类、二甲酚类等9种酚类物质迁移量同时测定的高效液相色谱（HPLC）检测方法。采用Agilent ZORBAX SB－Aq色谱柱进行分离，以甲醇－水为流动相梯度洗脱，流速1.0 mL/min，荧光检测器测定，激发波长为214 nm，发射波长为315 nm。优化了含油脂食品模拟物中9种酚类物质的提取方式，并考察了线性范围、准确度及精密度等方法性能参数。研究结果表明，含油脂食品模拟物中9种酚类物质的最佳提取溶剂为67%甲醇溶液，9种酚类物质在水基及油脂类食品模拟物中的线性相关系数（r2）均高于0.999，回收率为90.7%～99.8%，相对标准偏差均低于6.0%。5种水基食品模拟物中9种酚类物质的定量下限为0.07～0.10 mg/L，橄榄油中9种酚类物质的定量下限为0.15～0.20 mg/kg。方法成功应用于11批食品接触用聚碳酸酯（PC）塑料制品、15批涂层制品中9种酚类物质迁移量的测定。该方法灵敏度高、精密度好，可为食品接触材料及制品中9种酚类物质迁移量的分析提供技术支持。 |
| 中文关键词:酚类物质 迁移 食品接触材料 高效液相色谱法（HPLC） 荧光检测器 |
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| Determination of Migration of 9 Phenols in Food Contact Materials by High Performance Liquid Chromatography |
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| Abstract:A high performance liquid chromatographic（HPLC） method was established for the simultaneous determination of 9 phenols such as phenol，methyl-phenols and xylenols in food simulants. The sample was separated on an Agilent ZORBAX SB－Aq column by gradient elution，using methanol and water as mobile phase at a flow rate of 1.0 mL/min，and finally determined by fluorescence detection at an excitation wavelength of 214 nm and an emission wavelength of 315 nm．Meanwhile，the extraction method for 9 phenols in the fat-contained simulant was optimized，and the performances of the method including linearity，spiked recovery and precision were studied．Results demonstrated that 67% methanol aqueous solution was the best extraction solvent for the 9 phenols in fat simulants．The results also showed that there were good linear relationships for 9 phenols in all food simulants studied，with correlation coefficients（r2） higher than 0.999．The recoveries for 9 phenols at 3 spiked levels in all food simulants ranged from 90.7% to 99.8%，with relative standard deviations （RSD） all below 6.0%．The limits of quantitation（LOQs） were in the ranges of 0.07－0.10 mg/L for aqueous simulants，and 0.15－0.20 mg/kg for fat simulants. This method was successfully applied to the migration determination of 9 phenols released from 11 plastics articles（PC） and 15 coatings intended to contact with food．The method was sensitive and accurate，and was suitable for the determination of 9 phenols in food simulants. |
| Key Words:phenols migration food contact materials high performance liquid chromatography （HPLC） fluorescence detector |
| 引用本文：刘桂华，苏凤，李威，寇海娟，眭洁，李晓曼，罗世鹏.高效液相色谱法测定食品接触材料及制品中9种酚类物质的迁移量[J].分析测试学报,2021,40(11):1619-1626. |
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