| 基于CRISPR/Cas的生物传感平台在分子诊断中的应用 |
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| 基金项目:Natural Science Foundation of Henan Province of China（202300410145）；National Natural Science Foundation of China（22135004，21974006） |
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| 中文摘要:簇状规则间隔短回文重复序列（CRISPR）和CRISPR相关蛋白（Cas）不仅在基因工程领域炙手可热，而且正发展成为核酸精准检测领域的新利器。得益于Cas蛋白对核酸序列的特异性识别以及部分Cas蛋白的附属切割活性，Ⅱ类CRISPR/Cas系统在体外诊断及现场即时检测领域独具优势。该综述简要介绍了CRISPR/Cas系统的工作机理，重点总结了近3年基于不同信号读出方式的CRISPR/Cas生物传感平台在分子诊断领域中的应用。 |
| 中文关键词:CRISPR/Cas ?分子诊断 ?即时检测 ?生物传感 |
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| CRISPR/Cas Based Biosensing Platforms for Molecular Diagnosis |
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| Abstract:Clustered regularly interspaced short palindromic repeats（CRISPR） and CRISPR－associated proteins（Cas） have become highly promising candidates in the construction of biosensing systems and diagnostic devices，in addition to their roles as revolutionary genome engineering tools．For?in vitro diagnostics，the class Ⅱ CRISPR/Cas systems possess unique advantages，such as high detection sensitivity and specificity，and the feasibility for developing point－of－care diagnostic technology．This minireview briefly introduces the working mechanism of CRISPR/Cas systems and mainly focus on the various diagnostic methods based on the CRISPR/Cas for detecting different forms of the genetic molecules in recent three years according to different readout methods． |
| Key Words:CRISPR/Cas ?molecular diagnosis ?point－of－care testing ?biosensing |
| 引用本文：李甜，李娜.基于CRISPR/Cas的生物传感平台在分子诊断中的应用[J].分析测试学报,2022,41(4):520-528. |
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