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基于CRISPR/Cas的生物传感平台在分子诊断中的应用
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作者单位
李甜,李娜 1. 河南科技大学 基础医学院河南 洛阳 471023 2. 北京大学 化学与分子工程学院北京 100871 
基金项目:Natural Science Foundation of Henan Province of China(202300410145);National Natural Science Foundation of China(22135004,21974006)
中文摘要:簇状规则间隔短回文重复序列(CRISPR)和CRISPR相关蛋白(Cas)不仅在基因工程领域炙手可热,而且正发展成为核酸精准检测领域的新利器。得益于Cas蛋白对核酸序列的特异性识别以及部分Cas蛋白的附属切割活性,Ⅱ类CRISPR/Cas系统在体外诊断及现场即时检测领域独具优势。该综述简要介绍了CRISPR/Cas系统的工作机理,重点总结了近3年基于不同信号读出方式的CRISPR/Cas生物传感平台在分子诊断领域中的应用。
中文关键词:CRISPR/Cas  ?分子诊断  ?即时检测  ?生物传感
 
CRISPR/Cas Based Biosensing Platforms for Molecular Diagnosis
Abstract:Clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associated proteins(Cas) have become highly promising candidates in the construction of biosensing systems and diagnostic devices,in addition to their roles as revolutionary genome engineering tools.For?in vitro diagnostics,the class Ⅱ CRISPR/Cas systems possess unique advantages,such as high detection sensitivity and specificity,and the feasibility for developing point-of-care diagnostic technology.This minireview briefly introduces the working mechanism of CRISPR/Cas systems and mainly focus on the various diagnostic methods based on the CRISPR/Cas for detecting different forms of the genetic molecules in recent three years according to different readout methods.
Key Words:CRISPR/Cas  ?molecular diagnosis  ?point-of-care testing  ?biosensing
引用本文:李甜,李娜.基于CRISPR/Cas的生物传感平台在分子诊断中的应用[J].分析测试学报,2022,41(4):520-528.
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