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基于酶循环放大的DNA生物传感器用于检测食管鳞状细胞癌生物标志物蛋白PDGF-BB
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作者单位
刘霄凡,齐晨,王维才,李雪梅 临沂大学 化学化工学院山东省肿瘤标志物检测技术重点实验室山东 临沂 276005 
基金项目:The National Natural Science Foundation of China(21876074);Taishan Scholars Program of Shandong Province (tsqn201812101)
中文摘要:食管鳞状细胞癌(ESCC)患者发病早期由于体内的肿瘤标志物种类少且含量低,难以在患病早期发现疾病从而快速反应并有效治疗。因此,找到一种合适的标志物对其进行快速高效检测是研究者亟待解决的问题。血小板衍生生长因子(PDGF-BB)在恶性肿瘤的早期诊断中具有极其重要的地位。该文基于酶循环放大荧光光谱设计了一种用于PDGF-BB检测的DNA生物传感器,利用特定位点剪切酶,实现了信号放大,检出限由1 fmol/L降至1 amol/L,大大提高了检测的灵敏度。该方法通过检测血液中PDGF-BB浓度,从而实现对ESCC的简单、快速、高效诊断,在生物化学和医学应用上具有重要价值。
中文关键词:肿瘤标志物  ?DNA生物传感器  ?酶循环放大  ?荧光检测
 
Detection of Biomarker Protein PDGF-BB in Esophageal Squamous Cell Carcinoma Using a DNA Biosensor Based on Enzyme Cycle Amplification
Abstract:The condition of patients with esophageal squamous cell carcinoma(ESCC) is difficult to be found and effectively treated in the early stage of the disease due to the few types and low levels of tumor markers in the body. Therefore,it is the most urgent problem for researchers to find a suitable marker for rapid and efficient detection. Platelet derived growth factor-BB(PDGF-BB) is extremely important for the early diagnosis of malignancy. A DNA biosensor based on enzyme cycle amplification fluorescence spectroscopy was designed for PDGF-BB detection in this work,using a shearing enzyme that can cut the specified site to achieve the signal amplification,reducing the limit of detection from 1 fmol/L to 1 amol/L,greatly improving the sensitivity of detection. This method is of great value in biochemical and medical applications for its simple,rapid and efficient diagnosis and detection on esophageal squamous cell carcinoma.
Key Words:tumor markers  ?DNA biosensors  ?enzyme cycle amplification  ?fluorescence detection
引用本文:刘霄凡,齐晨,王维才,李雪梅.基于酶循环放大的DNA生物传感器用于检测食管鳞状细胞癌生物标志物蛋白PDGF-BB[J].分析测试学报,2022,41(4):632-638.
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