| 金－银双金属纳米簇@多壁碳纳米管－二氧化钛纳米材料为新型氧化还原探针构建电化学免疫传感器 |
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| 基金项目:四川省科技厅应用基础研究项目（2019YJ0307）；特色植物开发研究四川省高校重点实验室项目（TSZW2005，TSZW2004）；绿色催化四川省高校重点实验室开放基金（LYJ1802）；达州市科技局应用基础研究项目（18YYJC0002） |
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| 中文摘要:该研究合成了具有独特“珍珠项链”结构的金－银双金属纳米簇@多壁碳纳米管－二氧化钛纳米复合材料（Au－Ag NCs@CNTs－TiO2?NPs）作为信号标签、载体和信号放大器，构建了一种高灵敏“三明治”式电化学免疫传感器用于糖类抗原19－9（CA19－9）的检测。首先将具有过氧化物模拟酶特性的磁性Au－Fe3O4 NPs用于修饰电极，然后通过化学键合作用将anti－CA19－9抗体和BSA固载于电极表面，再通过特异性反应结合不同浓度的抗原和具有氧化还原活性和放大作用的Au－Ag NCs@ CNTs－TiO2 NPs/Ab2/BSA生物耦合物形成夹心免疫结构进行检测。结果表明，通过双重过氧化物模拟酶催化底液中的H2O2，有效地放大了响应信号，在最优条件下，CA19－9在0.01～200 U/mL浓度范围内线性关系良好，最低检出限为0.003 U/mL。采用标准加入法测得免疫传感器的回收率为94.6%～105%，相对标准偏差（RSD）为4.3%～6.5%，表明该免疫传感器有望用于临床血清样品中CA19－9的检测。 |
| 中文关键词:双金属纳米簇 ?协同作用 ?Au－Ag NCs@ CNTs－TiO2纳米复合材料 ?糖类抗原19－9 |
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| Electrochemical Immunosensor Based on Au－Ag Bimetallic Nanoclusters@CNTs－TiO2 Nanomaterials as Novel Redox Probes |
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| Abstract:A highly sensitive "sandwich" electrochemical immunosensor was constructed for the detection of carbohydrate antigen 19－9（CA19－9），by synthesizing a unique "pearl necklace" structure of gold-silver bimetallic nanoclusters multi-walled carbon nanotubes-titanium dioxide nanocomposites（Au－Ag NCs@CNTs－TiO2 NPs） used as a signal label，carrier and signal amplifier．Firstly，the magnetic Au－Fe3O4?NPs with peroxidase mimetic properties were used to modify the electrode，then the anti－CA19－9 antibody and BSA were immobilized on the electrode surface through chemical bonding，and subsequently，the antibody was bound by specific reaction．Finally，Au－Ag NCs@CNTs－TiO2 NPs/Ab2/BSA biological coupling with redox activity and amplification was combined to form a sandwich immune structure．The experimental results showed that the response signal was effectively amplified by using double peroxide analog enzyme to catalyze H2O2 in the substrate. Under the optimal experimental conditions，CA19－9 showed a good linearity in the concentration range of 0.01－200 U/mL，with a detection limit of 0.003 U/mL．The recoveries of the immunosensor ranged from 94.6% to 105%，with relative standard deviations（RSD） of 4.3%－6.5%，indicating that the immunosensor could be used for the detection of CA19－9 in clinical serum samples. |
| Key Words:bimetallic nanoclusters ?synergistic effect ?Au－Ag NCs@CNTs－TiO2?nanocomposites ?carbohydrate antigen 19－9 |
| 引用本文：黄小梅，邓祥，邓子禾.金－银双金属纳米簇@多壁碳纳米管－二氧化钛纳米材料为新型氧化还原探针构建电化学免疫传感器[J].分析测试学报,2022,41(5):675-681. |
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