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超高效液相色谱 - 四极杆 - 静电场轨道阱高分辨质谱快速鉴定左归降糖舒心方化学成分
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作者单位
黄雅兰,张艳玲,向琴,喻嵘,吴勇军,刘秀 1. 湖南中医药大学 第一附属医院湖南 长沙 410011 2. 湖南中医药大学 中医学院湖南 长沙 410208 3. 湖南中医药大学 科技处湖南 长沙 410208 4. 湖南中医药大学 研究生院湖南 长沙 410208 5. 湖南中医药大学 药学院湖南 长沙 410208 
基金项目:国家自然科学基金项目(82074400,82004185);湖南省科技重点研发计划项目(2020SK2101);湖南省研究生科研创新项目(CX20210692);湖南省教育厅科学研究项目(18A220,20K094,20B450);中医方证研究转化医学湖南省重点实验室资助(2018TP1021);湖南省自然科学基金项目(2018JJ3394);湖南省中医药科研计划项目(201931)
中文摘要:建立了一种基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱技术(UHPLC-Q-Exactive-Orbitrap-MS)的左归降糖舒心方化学成分的快速分析方法。左归降糖舒心方水煎液高速离心后取上清液,以Xbridge BEH C18色谱柱(2.1 mm × 100 mm,2.6 μm)分离,0.1%甲酸水和乙腈为流动相进行梯度洗脱,采用四极杆-静电场轨道阱高分辨质谱在电喷雾正、负离子模式下进行检测,Xcalibur 4.3和Compound Discoverer 3.2软件进行数据处理。从左归降糖舒心方水煎液中共鉴定出290个化学成分,包括224个已知的化学成分,涵盖黄酮类、苯丙素类、酚类、皂苷类、有机酸类、生物碱类、核苷类、环烯醚萜类、寡糖等9种主要结构类型,此外66个化合物为新发现的成分,主要为氨基酸/小肽类结构类型。该方法可快速、准确地鉴定左归降糖舒心方水煎液中的化学成分,为进一步研究左归降糖舒心方的药效物质基础,指导临床合理用药以及后期实验研究提供参考依据。
中文关键词:左归降糖舒心方  超高效液相色谱 - 四极杆 - 静电场轨道阱高分辨质谱  化学成分  小肽类
 
Rapid Analysis of Chemical Components in Zuogui Jiangtang Shuxin Formula by UHPLC - Q - Exactive - Orbitrap Mass Spectrometry
Abstract:An ultra performance liquid chromatography-quadrupole-exactive orbitrap mass spectrometry(UHPLC-Q-Exactive-Orbitrap-MS)was developed for the rapid analysis of the chemical components in Zuogui Jiangtang Shuxin formula.The aqueous decoction of Zuogui Jiangtang Shuxin formula was separated on an Xbridge BEH C18(2.1 mm × 100 mm,2.6 μm) column with 0.1% formic acid-water and acetonitrile as mobile phase.The eluent was detected by quadrupole orbitrap high resolution mass spectrometry in ESI positive and negative ion modes.The raw data was processed using Xcalibur 4.3 and Compound Discoverer 3.2 software.A total of 290 chemical components were tentatively identified from the aqueous decoction of Zuogui Jiangtang Shuxin formula,including 224 known compounds with structure classes of flavonoids,phenylpropanoids,phenols,saponins,organic acids,alkaloids,nucleosides,iridoids and oligoses.It is worth noting that the other 66 compounds were detected and identified from Zuogui Jiangtang Shuxin formula for the first time,which were mainly amino acids/small peptides.This developed method could be used for rapid and accurate analysis of the chemical components in Zuogui Jiangtang Shuxin formula,which could provide a reference for further research on the therapeutic material basis of Zuogui Jiangtang Shuxin formula and guiding the rational use of drugs in clinical practice as well as later experimental studies.
Key Words:Zuogui Jiangtang Shuxin formula  ultra performance liquid chromatography-quadrupole-exactive orbitrap mass spectrometry  chemical components  small peptides
引用本文:黄雅兰,张艳玲,向琴,喻嵘,吴勇军,刘秀.超高效液相色谱 - 四极杆 - 静电场轨道阱高分辨质谱快速鉴定左归降糖舒心方化学成分[J].分析测试学报,2022,41(7):963-977.
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