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UPLC-ESI-Q-TOF MS结合裂解规律分析姜科植物中二苯基庚烷类成分
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作者单位
胡雪雨,王彦志,宋志敏,刘煜飞,刘双晶,冯卫生 1. 河南中医药大学 药学院河南 郑州 450046 2. 呼吸疾病中医药防治省部共建协同创新中心 河南 郑州 450046 
基金项目:河南省自然科学基金面上项目(212300410368);河南省中医学科研究专项课题(20-21ZY1040)
中文摘要:根据标准品的质谱数据,探讨二苯基庚烷类成分的裂解规律,并利用裂解规律分析姜科植物中的该类成分。采用针泵注射进样,利用高分辨率和高准确性的电喷雾-四极杆-飞行时间质谱(ESI-Q-TOF MS)得到正离子模式下(ESI+)11个标准品的质谱数据。在此基础上,分析标准品的裂解行为,归纳4类二苯基庚烷类化合物的裂解途径,总结出二苯基庚烷类化合物的裂解规律。据此建立快速识别二苯基庚烷类成分的方法,结合超高效液相色谱-电喷雾-四极杆-飞行时间串联质谱(UPLC-ESI-Q-TOF MS),对姜科植物来源中药姜皮、草果、益智仁中的二苯基庚烷类成分进行识别,从中分别鉴定出14、11、10个此类成分。结果表明,该方法准确、可靠、高效,可快速鉴定和分析姜科植物中的二苯基庚烷类化合物。
中文关键词:二苯基庚烷  裂解规律  UPLC-ESI-Q-TOF MS  姜科植物
 
Analysis of Diarylheptanoids in Zingiberaceae by UPLC- ESI-Q-TOF MS Combined with Fragmentation Rule
Abstract:According to the mass spectrum data of the standard substance,the fragmentation rule of diarylheptanoid was discussed,and the component in zingiberaceae plants was analyzed by the fragmentation rule.The standard samples were injected by syringe pump.The mass spectrum data of 11 standards were obtained by high resolution and high accuracy electrospray ionization quadrupole time-of-flight tandem mass spectrometry(ESI-Q-TOF MS) in positive ion mode(ESI+).On the basis of the obtained standards mass spectrum data,the cracking behaviors of the standards were analyzed.According to the cracking behaviors of standard samples,four kinds of cracking pathways of diarylheptanoid were summarized,and the fragmentation rule of diarylheptanoid was summarized.Based on the fragmentation rule of diarylheptanoid standards,an ultra-high performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry(UPLC-ESI-Q-TOF MS) was established for the rapid identification of diarylheptanoid components in ginger peel,Amomum tsao-ko and Alpinia oxyphylla Miq. 14, 11 and 10 of this kind of components were successfully identified from them,respectively.The obtained results verified the feasibility of this method.The method is accurate,reliable and efficient,and it could be used for rapid identification and analysis of diarylheptanoids in zingiberaceae plants.
Key Words:diarylheptanoids  fragmentation rule  UPLC-ESI-Q-TOF MS  zingiberaceae
引用本文:胡雪雨,王彦志,宋志敏,刘煜飞,刘双晶,冯卫生.UPLC-ESI-Q-TOF MS结合裂解规律分析姜科植物中二苯基庚烷类成分[J].分析测试学报,2022,41(7):1036-1044.
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