| 单细胞与空间转录组分析研究进展 |
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| 基金项目:国家自然科学基金资助项目（21927806，21735004，21521004，21325522，21904085） |
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| 中文摘要:单细胞的异质性在胚胎发育、神经系统发育、癌症病变等生物学过程中具有重要的研究意义。单细胞转录组测序利用测序技术解析细胞内转录本序列，为细胞异质性研究提供可靠、准确的研究手段及观测视野，为揭示生物发育规律、疾病发生发展机制等提供了重要的技术手段。该文围绕单细胞与空间组学分析，综述了近年来发展的各类单细胞转录组测序、单细胞多组学测序及空间转录组分析方法，分析了其优缺点。同时展望了单细胞与空间转录组分析领域的发展趋势，即开发低成本、全方位、高通量、高分辨的单细胞空间多组学分析方法以实现对单个细胞更为细致、全面及准确的描述和精准单细胞图谱构建。 |
| 中文关键词:单细胞测序 转录组 多组学 空间转录组 研究进展 |
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| Progress on Single-cell and Spatial Transcriptome Analysis |
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| Abstract:Heterogeneity in single cells has important implications for embryo development，neurogenesis and cancer progress．Single-cell transcriptome sequencing，decoding transcript sequences within cells based on high-throughput sequencing technology，can provide reliable and accurate research methods for cellular heterogeneity study to reveal the rule of biological development and disease progress．Different methods for single-cell transcriptome sequencing，single-cell multi-omics sequencing and spatial transcriptome analysis in recent years are summarized in this review．Challenges and perspectives of single-cell and spatial transcriptome analysis are discussed．Low-cost，all-round，high-throughput，high-resolution single-cell spatially multi-omics analysis is helpful to construct comprehensive and accurate single-cell atlas. |
| Key Words:single-cell sequencing transcriptome multi-omics spatial transcriptome research progress |
| 引用本文：许醒，蔡林峰，张倩楠，杨启正，梁珊珊，李铭殷，李翀，杨朝勇.单细胞与空间转录组分析研究进展[J].分析测试学报,2022,41(9):1322-1334. |
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