•  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
期刊检索


检索
检索项:
检索词:
总目录
  • WCSB9︱2019(第九届)世界采样和混样大会 查看全文>>
  • 关于召开第十五届全国青年分析测试学术报告会的通知 查看全文>>
友情链接
DNA脱碱基位点的检测方法及其生物学研究进展
    点此下载全文
作者单位
武海江,张雅姣,袁舒妍,徐华,谢剑炜 1. 军事医学研究院毒物药物研究所抗毒药物与毒理学国家重点实验室北京 100850 2. 沈阳药科大学 无涯创新学院辽宁 沈阳 110016 
基金项目:国家自然科学基金资助项目(22104153)
中文摘要:DNA中糖苷键断裂形成的脱碱基位点(脱嘌呤/嘧啶位点,AP位点)是常见的DNA损伤类型之一,由核苷酸自发水解产生,也是DNA碱基切除修复途径中的关键中间体。若修复不及时,可能会导致DNA复制阻滞和DNA链断裂,产生突变和细胞毒性,同时还会引起形成DNA交联或DNA-蛋白质交联的损伤,因此对于AP损伤的检测有助于理解细胞氧化应激损伤和基因毒性物质的毒性评价。目前检测AP位点的方法有14C或32P后标记法、酶联免疫吸附分析(ELISA)法和液相色谱-质谱(LC-MS)技术等。该文重点概述DNA中AP位点的检测方法和生物学研究进展,并展望了AP位点损伤相关研究的前景。
中文关键词:脱碱基位点  DNA损伤  质谱  DNA链间交联  DNA-蛋白质交联
 
Research on Detection Methods and Biology of DNA Abasic Sites
Abstract:The abasic sites(apurinic/apyrimidinic sites,AP sites) generated from the cleavage of N-glycosylic bond in DNA are one of the common lesions in DNA,which are formed via spontaneous hydrolysis,as well as a major intermediate during the base excision repair.If AP sites are not repaired timely,they may cause DNA replication stalling and DNA strand breaks,resulting in mutagenesis and cytotoxicity,and further cause DNA crosslinks or DNA-protein crosslinks.Therefore,analysis of AP sites will be helpful for the assessment of cellular oxidative stress damage or the toxicity evaluation of genotoxic chemicals.A variety of analytical methods have been developed for the detection of AP sites in DNA,including 14C or 32P postlabeling,enzyme-linked immunosorbent assay(ELISA) and liquid chromatography-mass spectrometry(LC-MS).The research progress in the detection methods and biology of AP sites in DNA is reviewed in this paper,and the research prospects of AP sites are presented.
Key Words:abasic sites  DNA damage  mass spectrometry  DNA interstrand crosslink  DNA-protein crosslink
引用本文:武海江,张雅姣,袁舒妍,徐华,谢剑炜.DNA脱碱基位点的检测方法及其生物学研究进展[J].分析测试学报,2022,41(9):1365-1374.
摘要点击次数: 300
全文下载次数: 458
查看全文  下载PDF阅读器