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膜蛋白质复合物提取方法研究进展
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作者单位
褚宏伟,郑诗颖,赵群,赵宝锋,梁振,张丽华,张玉奎 1. 中国科学院大连化学物理研究所中国科学院分离分析化学重点实验室国家色谱研究分析中心 辽宁 大连 116023 2. 大连理工大学 张大煜学院辽宁 大连 116024 3. 中国科学院大学北京 100049 
基金项目:国家自然科学基金项目(21834006)
中文摘要:膜蛋白质通过与其他蛋白质相互作用形成膜蛋白质复合物(Membrane protein complexes,MPCs)形式来行使重要的生物学功能,譬如参与细胞-细胞交流、信号传导与分子识别等。MPCs的功能异常会导致多种疾病的发生和临床治疗耐药等问题。研究完整MPCs的组装对于绘制全景式蛋白质-蛋白质相互作用网络,进而揭示膜蛋白质机器的功能具有重要意义。然而,由于MPCs的过表达和纯化仍面临巨大挑战,如何从细胞中实现MPCs的高效提取尤为重要。因此,亟需发展既可确保MPCs提取效率,并能保持其结构稳定性的提取方法。该文对现有MPCs提取方法及其应用进展进行了综述,并对其发展前景加以展望,以期为MPCs的功能解析提供技术支撑。
中文关键词:膜蛋白质复合物  蛋白质-蛋白质相互作用  高效提取  结构稳定
 
Advances on Extraction Methods of Membrane Protein Complexes
Abstract:Membrane proteins are able to carry out important biological functions attributable to the formation of membrane protein complexes(MPCs),which takes place when they collaborate with other proteins.These functions include participating in the communication between cells,signal transduction,and the recognition of molecular targets.The dysfunction of MPCs can lead to the occurrence of various diseases and clinical drug resistance.Investigation on the formation of intact MPCs is of great importance for both the mapping of a comprehensive protein-protein interaction network and the subsequent understanding of the function of membrane protein machinery.The overexpression and purification of MPCs still face significant challenges.Hence,how the MPCs are to be extracted from cells efficiently is of critical importance.As a result,there is an urgent need for the development of an extraction method which is capable of both ensuring the extraction efficiency for MPCs and simultaneously maintaining their structural stability.In this paper,the existing MPCs extraction methods and their application progress are reviewed,and their development prospects are prospected,in order to provide a technical support for the functional analysis of MPCs.
Key Words:membrane protein complexes  protein-protein interactions  efficient extraction  structural stability
引用本文:褚宏伟,郑诗颖,赵群,赵宝锋,梁振,张丽华,张玉奎.膜蛋白质复合物提取方法研究进展[J].分析测试学报,2022,41(9):1387-1394.
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