| 啤酒基荧光碳点的绿色合成及其在Fe3+检测和细胞成像中的应用 |
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| 基金项目:国家自然科学基金资助项目（22006143，21806189） |
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| 中文摘要:以啤酒为碳源，通过水热法合成了一系列啤酒基荧光碳点，其中以齐鲁工业大学自酿啤酒合成的碳点相比其他品牌啤酒合成的碳点具有更好的发光效率。该碳点表现出发射波长随激发波长变化而变化的性质，最大发射波长为475 nm，呈现蓝色荧光。利用透射电子显微镜、X射线衍射技术、傅里叶变换红外光谱、紫外－可见吸收光谱和荧光光谱等对碳点结构及其光学特性进行了表征。基于Fe3+对碳点的特异性荧光猝灭，建立了灵敏的Fe3+检测方法，线性范围为0.3 ~ 45 μmol/L，检出限达91 nmol/L，并成功用于实际水样中Fe3+浓度的分析。所合成的碳点具有良好的生物相容性和细胞膜穿透性，可用于细胞内Fe3+的可视化成像分析和多色细胞成像。 |
| 中文关键词:碳点 水热合成 啤酒 Fe3+检测 细胞成像 |
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| Green Synthesis of Beer-based Fluorescent Carbon Dots and Their Application in Fe3+ Detection and Cell Imaging |
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| Abstract:Ferric ion with an abnormal concentration level can cause obvious body dysfunction，and even lead to Parkinson's disease，Alzheimer's disease and other serious diseases．Therefore，it is very important to develop an effective analytical method for the detection of Fe3+ in environmental samples and the dynamic monitoring of Fe3+ in cells for the maintenance of normal physiological function of human body．In this study，a series of beer-based fluorescent carbon dots（CDs） were synthesized by hydrothermal method using beers as carbon sources．The CDs synthesized with the self-brewed beer of Qilu university of technology had better luminous efficiency than those synthesized with other brands．The structure and optical properties of CDs were characterized by transmission electron microscopy（TEM），X-ray diffraction（XRD），Fourier transform infrared spectroscopy（FTIR），UV-Vis absorption spectroscopy（UV－Vis） and fluorescence spectroscopy．The results showed that the CDs were spherical monodispersed nanoparticles with a relatively narrow size distribution and an average diameter of 5.6 nm，which had an amorphous carbon structure and contained a large number of active functional groups such as C—N/C—O and C? O on the surface. The CDs were determined to be composed of carbon，nitrogen and oxygen by X-ray photoelectricity spectroscopy．The CDs shows the property that the emission wavelength changes with the excitation wavelength，and the maximum emission wavelength is 475 nm，showing blue fluorescence．At the excitation wavelength of 400 nm，it has stable fluorescence in the pH range of 3.0－12.0．The effects of UV irradiation time and salt concentration on fluorescence intensity were investigated．The fluorescence intensity decreased by less than 6% even after 6 h UV irradiation．When the salt concentration was up to 0.5 mol/L，the fluorescence intensity did not change significantly．Therefore，the CDs has good optical stability．Based on the specific fluorescence quenching of CDs by Fe3+，a sensitive Fe3+ detection method was established．The linear range was 0.3－45 μmol/L，the correlation coefficient was 0.998 6，and the detection limit was 91 nmol/L．Common metal ions did not cause significant interference．The method has been successfully applied to the concentration analysis of Fe3+ in tap water，rain water and spring water．The spiked recoveries range from 94.6% to 106%，and the relative standard deviation is less than 5%．Hela cells were used as a model to study the cytotoxicity of CDs by MTT assay．The results showed that the synthesized CDs had good biocompatibility and cell membrane penetration，and were further used for visualization analysis of intracellular Fe3+ and multicolor cell imaging．This study provides a new idea for promoting the green synthesis of CDs and the development of new analytical methods. |
| Key Words:carbon dots hydrothermal synthesis beer Fe3+ detection cell imaging |
| 引用本文：胡金双，杨熙，刘舒芹，杨升宏.啤酒基荧光碳点的绿色合成及其在Fe3+检测和细胞成像中的应用[J].分析测试学报,2022,41(11):1644-1651. |
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