| 基于超高效液相色谱－四极杆－静电场轨道离子阱质谱的羊乳脂肪球膜全蛋白谱泌乳期时序差异研究 |
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| 基金项目:国家自然科学基金（32272401）；咸阳市科学技术研究计划项目（2021ZDYF-NY-0025）；西安市科技计划项目（21NYYF0056）；陕西省创新能力支撑计划资助项目（2021KJXX-37） |
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| 中文摘要:该文利用高通量质谱结合非标记蛋白质定量技术对不同泌乳期羊乳的乳脂肪球膜蛋白质（MFGM）进行鉴定，在羊初乳和羊常乳中分别定量到331和252种乳脂肪球膜蛋白质，其中164种蛋白质在两组乳样中共同表达。对所鉴定脂肪球膜蛋白质进行单变量及多变量分析，建立偏最小二乘判别分析模型，分别以2、0.05和1.2为倍数变化、显著性差异及变量投影重要性分析值的阈值，筛选羊初乳和羊常乳中的差异性标志物。经生物信息学分析发现，鉴定的蛋白质标志物主要参与炎症过程、整合素信号通路及半乳糖代谢过程。研究结果对提高对羊乳蛋白质的深入认识与羊乳及其制品的营养改良具有重要意义。 |
| 中文关键词:羊乳 乳脂肪球膜蛋白质（MFGM） 超高效液相色谱－四极杆－静电场轨道离子阱质谱 蛋白质组学 |
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| Study on Timing Difference of Goat Milk MFGM Protein in Lactation Period Based on Ultrahigh Performance Liquid Chromatography－Quadrupole－Orbitrap Mass Spectrometry |
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| Abstract:High throughput mass spectrometry combined with label-free quantitative technique was used to identify the milk fat globule membrane（MFGM） proteins of goat milk at different lactation stages．331 and 252 MFGM proteins were quantified in goat milk colostrum and mature milk，respectively，of which 164 proteins were co-expressed．The identified MFGM proteins were analyzed by univariate and multivariate analysis，and the partial least squares discriminant analysis model was established. Taking 2，0.05 and 1.2 as the thresholds of fold change，significant difference and variable projection importance analysis value，the differential markers in colostrum and mature milk were screened．Bioinformatics analysis showed that the identified protein markers mainly involved in inflammatory process，integrin signaling pathway and galactose metabolism．The results were of great significance to facilitate people’s in-depth understanding of goat milk protein，upgrading the nutritional improvement of milk and its products and the production of breast milk substitutes. |
| Key Words:goat milk milk fat globule membrane（MFGM） proteins ultrahigh performance liquid chromatography－quadrupole－orbitrap mass spectrometry proteomics |
| 引用本文：张荣，贾玮.基于超高效液相色谱－四极杆－静电场轨道离子阱质谱的羊乳脂肪球膜全蛋白谱泌乳期时序差异研究[J].分析测试学报,2022,41(12):1722-1728. |
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