| 高效液相色谱法同时测定化妆品中辣椒碱等9种热感剂 |
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| 中文摘要:建立了测定化妆品中辣椒碱等9种热感剂的高效液相色谱法。样品经80%甲醇超声提取后，过滤，以乙腈-0.5%甲酸水溶液为流动相，采用SymmetryShieldTMRP18色谱柱（250 mm × 4.6 mm，5 μm）分离，液相色谱分离，二极管阵列检测器进行检测。在优化实验条件下，9种热感剂的分离度好，专属性高，其质量浓度在0.2 ~ 100 μg/mL范围内与峰面积呈良好线性关系，相关系数（r2）均不小于0.999 5，检出限（LOD）为0.001 0 ~ 0.003 4 mg/kg。以水基类、乳剂类、膏霜类3种阴性样品作为空白基质，9种热感剂在0.004、0.02、1 mg/kg 3种加标水平下的回收率为75.6% ~ 115%，相对标准偏差（RSD）为0.083% ~ 7.0%。该方法专属性强、灵敏度高、准确度好，可用于化妆品中9种热感剂含量的测定。 |
| 中文关键词:热感剂 化妆品 高效液相色谱法（HPLC） |
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| Simultaneous Determination of 9 Self-heating Compounds Including Capsaicin in Cosmetics by High Performance Liquid Chromatography |
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| Abstract:A high performance liquid chromatographic（HPLC） method was established for the determination of 9 self-heating compounds，including capsaicin in cosmetics．The samples were ultrasonically extracted with 80% methanol，and then filtered．The target compounds were separated on a SymmetryShieldTMRP18（250 mm × 4.6 mm，5 μm） with a mobile phase consisting of acetonitrile and 0.5% formic acid by gradient elution，and analyzed by HPLC with a PDA detector．Under the optimal conditions，good linear relationships were obtained for nine compounds in the concentration range of 0.2－100 μg/mL，with correlation coefficients（r2） not less than 0.999 5．The limits of detection（LODs） were in the range of 0.001 0－0.003 4 mg/kg．The spiked recoveries for water，lotion and cream matrixes at three levels of 0.004，0.02，1 mg/kg ranged from 75.6% to 115%，with relative standard deviations（RSDs，n = 3） of 0.083%－7.0%．This method is accurate and sensitive，and is suitable for the qualitative and quantitative analysis of 9 self-heating compounds in cosmetics. |
| Key Words:self-heating compounds cosmetics high performance liquid chromatography（HPLC） |
| 引用本文：吴志珊，吴姣娇，尹伟成，殷帅，李卓芳，姜成君.高效液相色谱法同时测定化妆品中辣椒碱等9种热感剂[J].分析测试学报,2023,(5):641-646. |
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