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| Determination of 18 Mycotoxin Contaminants in Peanuts and Oils by Gel Permeation Chromatography and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS) gel permeation chromatography（GPC) isotope internal standard mycotoxins |
| Author | Institution |
| GONG Xiaoming1，REN Yiping2，DONG Jing1，SUN Jun1，LI Jian3，JIN Chao1，YU Jinling1 |
1.潍坊出入境检验检疫局;2.浙江省疾病预防控制中心;3.中国水产科学研究院黄海水产研究所 |
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| Abstract: |
| A method for the analysis of common mycotoxins in peanuts and oils was developed using ultra performance liquid chromatography with tandem mass spectrometry（UPLC-MS/MS)，based on isotope dilution and gel permeation chromatography（GPC) purification.The homogenized sample was spiked with U-［13C17］-aflatoxin B1 and U-［13C15］-deoxynivalenol)，and extracted with acetonitrile-water（84∶16，by volume)，then purified by GPC.The targeted compounds were detected by MS/MS system with eletrospray ionization（ESI) under muti reaction monitoring（MRM) mode and quantified by the internal standard calibration curve of isotope dilution technique.The result indicated that the LOQ of 12 positive ions ranged from 002 μg/kg to 04 μg/kg，the LOQ of 6 negative ions ranged from 006 μg/kg to 10 μg/kg.Meanwhile，the high correlation coefficents（r≥0996) of 18 mycotoxins were obtained within their respective linear ranges.The average recoveries at low,intermediate and high spiked levels ranged from 80% to 96% with relative standard deviations（RSD) of 105%-196%.This method was suitable for the identification and quantification of multi component mycotoxin contaminants in complex matrixes with advantages of simple pretreatment,good purification and high sensitivity. |
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