Simultaneous Determination of 9 Sulfonylurea Herbicides in Corn Using Solid phase Extraction and Ultra Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry
  
View Full Text    Download reader
DOI:10.3969/j.issn.1004-4957.年份.月份
KeyWord:SPE  ultra performance liquid chromatography-tandem mass spectrometry  sulfonylurea herbicides  corn
  
AuthorInstitution
DING Fei1, 2, LI Fanzhu1, CHU Xiaogang2, ZHANG Feng2, LING Yun2, SUN Li2, YANG Minli2, L Quanfu2,3, XU Chengbao2 1.浙江中医药大学药学院;2.中国检验检疫科学研究院;3.西南大学药学院
Hits: 2431
Download times: 1457
Abstract:
      A new method based on solid phase extraction and ultra performance liquid chromatography-tandem mass spectrometry(UPLC-ESI MS/MS) was developed for the determination of 9 sulfonylurea herbicides(metoxuron, foramsulfuron, cinosulfuron, methabenthiazuron, prosulfuron, linuron, ethoxysulfuron, chloroxuron and cyclosulfamuron) in corn. The corn sample was soared in water, and then extracted with methanol-acetone (1∶1, by volume). The extract was cleaned up by C18 solid phase extraction cartridge. The eluent was evaporated to near dryness under a stream of nitrogen gas, and redissolved by methanol. The identification and detection of target compounds were achieved under multiple reactions monitoring (MRM)mode with positive electrospray ionization(ESI). 9 sulfonylurea herbcides were quantified by external standard method. The calibration curves of 9 sulfonylurea herbicides were linear in the range of 005-20 mg?L-1 with correlation coefficients more than 099. The limits of detection (LOD, S/N=3) and the limits of quantitation (LOQ, S/N=10) were in the range of 0001-0005 mg?L-1 and 0062-0080 mg?kg-1, respectively. 1LOQ, 2LOQ and 5LOQ were chosen as the three spiked levels of recovery for the method. The recoveries of 9 sulfonylurea herbicides ranged from 60% to 85% with relative standard deviations less than 10%. The method was simple and effective, and could meet the requirements the simultaneous determination of 9 sulfonylurea herbicides in corn sample.
Close