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| Simultaneous Determination of 22 Food Additives in Carbonated Beverages by Ultra High Performance Liquid Chromatography |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:ultra performance liquid chromatography（UPLC） carbonated beverage food additive determination |
| Author | Institution |
| 黄永辉，余清，林钦，黄红霞，谢勇，梁敏 |
福建省产品质量检验研究院 |
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| Abstract: |
| A simple and rapid method was developed for the simultaneous determination of 22 food additives in carbonated beverages by ultra high performance liquid chromatography（UPLC）.The sample was degassed by ultrasonic and added 10% ethanol.The pH was regulated to 8.0 using 25% ammonira-water （1∶1）.The separation was achieved on a BEH C18 column（1.7 μm，2.1 mm×50 mm） in 15 min by using 0.01 mol/L NH4Ac（pH 5.8-6.0） and acetonitrile as mobile phase with a flow rate of 0.2 mL/min at 35 ℃.
A diode array detector was used to detect at 217,232 and 257 nm.The quantification was performed by the external standard method.The method showed good linearities for 22 food additives with correlation coefficients（r） more than 0.995.The detection limits ranged from 0.02 mg/L to 0.50 mg/L.The average recoveries for 22 food additives from spiked carbonated beverage samples were in the range of 81%-107% with relative standard deviations（RSDs）of 1.0%-12.9%.With the advantages of simplicity,sensitivity and repeatability,the method could meet the requirements for determination of food additives in carbonated beverages at home. |
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