Separation and Determination of Saikosaponin a and Saikosaponin d of Bupleuri Radix by Capillary Electrophoresis
  
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DOI:10.3969/j.issn.1004-4957.年份.月份
KeyWord:high performance capillary electrophoresis(HPCE)  Bupleuri Radix  saikosaponin a  saikosaponin d
  
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李艺,易润青,刘丹,宋粉云 广东药学院药科学院
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Abstract:
      A high performance capillary electrophoresis(HPCE) method was established for the separation and quatitative determination of saikosaponin a and saikosaponin d in Bupleuri Radix by using sulfobutyl ether-β-cyclodextrin(SBE-β-CD)and β-cyclodextrin(β-CD) as dual cyclodextrin(CD) system.Experimental conditions,such as the concentration of running buffer,the types and concentration of CDs,separation voltage and sample medium,were investigated.The results indicated that saikosaponin a and saikosaponin d could be baseline separated on an uncoated fused sillica capillary column(60 cm×50 μm i.d.,effective length:53 cm) using 20 mmol/L tris(hydroxymethyl)aminomethane-20 mmol/L SBE-β-CD-25 mmol/Lβ-CD(pH 9.9) as running buffer.The separation voltage was set at 15 kV and the detection wavelength was 210 nm.Samples were injected into capillary by gravity for 10 s and p-nitrobenzoic acid was used as the internal standard.Under the optimal conditions,the calibration curves of saikosaponin a and saikosaponin d were linear in the concentration ranges of 60-300 mg/L(r=0.999 7) and 20-100 mg/L (r=0.999 5),respectively.The average recoveries of saikosaponin a and saikosaponin d were 102% and 100%,respectively,with RSD of 1.2% and 2.3%.The HPCE method was convenient,rapid and well repeatable,and was suitable for the quantitative analysis of saikosaponin a and saikosaponin d in Bupleuri Radix.
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