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| Investigation on Interaction of Bovine Serum Albumin with ZnS Nanoparticles by Fluorescence Spectroscopy |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:Nano-ZnS bovine serum albumin（BSA） fluorescence spectroscopy interaction static quenching |
| Author | Institution |
| 陈稚，吴都督，张爽，林晓，龙烁杭，施志海，吴锐彬 |
1.广东医学院分析中心;2.广东医学院药学院;3.广东医学院医学检验学院 |
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| Abstract: |
| ZnS nanoparticles was synthesized using zinc acetate and sodium sulfide as starting materials in water solution.The morphology and crystal phase of nano-ZnS were characterized by transmission electron microscopy（TEM） and powder X-ray diffraction（XRD）.The interaction of nanoparticles and bovine serum albumin（BSA） was investigated by fluorescence spectroscopy in pH 7.0 Tris-HCl buffer.The result showed that the interaction between BSA and ZnS nanoparticles could resulted in the endogenous fluorescence quenching of BSA,which belonged to a static quenching mechanism.The quenching rate constant was 1.88×1014 L?mol-1?s-1,the binding constant（Ka） and binding site（n） were 1.73×105 L?mol-1 and 0.83,respectively. |
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