Determination of Silicate in Cyclotella Meneghiniana Kiits Solution Using Non suppressed Conductance Detection Ion Chromatography
  
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DOI:10.3969/j.issn.1004-4957.年份.月份
KeyWord:ion chromatography  non-suppressed conductance detection  silicate  diatom
  
AuthorInstitution
李月,刘晃 农业部渔业装备与工程技术重点实验室中国水产科学研究院渔业机械仪器研究所
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Abstract:
      A novel method was developed for the determination of SiO2-3 in Cyclotella meneghiniana Kiits solution by non-suppressed conductance detection ion chromatography.The chromatography was performed on a Dionex anion AS23(4 mm×250 mm) analytical column and AG23(4 mm×50 mm) guard column with a constant temperature conductance detector.The experimental conditions were optimized,and the optimal chromatographic conditions were using 4 mmol/L sodium carbonate as eluent with a eluent speed of 1.0 mL/min,injection sample volume of 100 μL.The calibration curve of silicate was linear in the range of 0-40 mg/L.The detection limit was 0.017 mg/L.The relative standard deviation(RSD,n=7) for standard solution was lower than 5%.The RSDs for peak area and peak height measurements were 10.9% and 4.8%,respectively.The proposed method was applied in the determination of silicate in Cyclotella meneghiniana Kiits solution in different growing periods,and the spiked recoveries were in the range of 102%-120%.With the advantages of simplicity,good sensitivity,low cost,the method was suitable for the determination of silicate in cyclotella meneghiniana kiits solution.
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