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| Separation of Three Single Phosporic Acidic Adenosines by a Nonaqueous Capillary Electrophoresis Method and Its Application |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:nonaqueous capillary electrophoresis(NACE) single phosporic acidic adenosine nucleoside separation |
| Author | Institution |
| 宋娟梅，成光，边志明，贾鹏 |
1.淄博市环境监测站;2.淄博建筑工程学校;3.郑州大学化学系 |
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| Abstract: |
| The separation of 2′-AMP，3′-AMP and 5′-AMP were investigated by a nonaqueous capillary electrophoresis（NACE) method.Effects of pH* value，nonaqueous solvent，different category buffer on the separation efficiency were studied.The optimum separation conditions are as follows:running medium:200 mmol?L-1 Tris-H3BO3 containing 50% acetonitrile，pH* value:10.0; applied electric field:25 kV; column temperature:25 ℃; detection wavelength:260 nm，and samples were injected from the cathodal end of the capillary by 50 mbar for 5 s.Under the optimal conditions，there are excellent linearities between peak heights and concentrations of the analytes in the concentration range of 1-100 mg/L.The spiked recoveries ranged from 88% to 106% with RSDs less than 4%.The method was applied in the determination of adenosine samples with satisfactory results. |
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