|
| Study on the Hydrophilic Interaction Chromatography Specific Fingerprint of Mulberry leaves |
| |
| View Full Text Download reader |
| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:mulberry leaves hydrophilic interaction chromatography fingerprint |
| Author | Institution |
| 东莎莎，赵恒强，王晓，崔清华，耿岩玲，刘代成，刘建华，王小如 |
1.山东师范大学生命科学学院;2.山东省科学院中药过程控制研究中心，山东省大型精密分析仪器应用技术重点实验室，山东省分析测试中心;3.国家海洋局第一海洋研究所，青岛市现代分析技术及中药标准化重点实验室;4．山东中医药大学药学院 |
|
| Hits: 3139 |
| Download times: 1755 |
| Abstract: |
| A hydrophilic interaction chromatography(HILIC) fingerprinting method had been established for the quality evaluation and discrimination of mulberry leaves.The chromatographic fingerprints were determined by injecting 20 μL of the sample solution each time into the hydrophilic interaction chromatography column(XBridgeTM Amide column) with the gradient elution solvent system composed of water(containing 0.2% formic acid,20 mmol/L ammonium formate and 20% methanol) and acetonitrile．The flow rate was 0.8 mL/min,the column temperature was maintained at 25 ℃ and the signals were acquired at 322 nm.The result indicated that the developed HILIC method was simple,accurate and reliable for the development of mulberry leaves fingerprint.The HILIC fingerprints for ten samples tested contained the same seventeen peaks and four characteristic peaks.Combining with the similarity analysis, the method could be used to discriminate different origins of mulberry leaf samples.The HILIC fingerprinting technique is expected to become an important tool in the identification and quality evaluation of mulberry leaves. |
| Close |