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| Determination of Ellagic Acid and Punicalagin in Pomegranate Peel by Capillary Electrophoresis |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:capillary electrophoresis pomegranate peel ellagic acid punicalagin |
| Author | Institution |
| 纪白慧, 杨笑笑,倪鑫炯,曹玉华 |
江南大学化学与材料工程学院 |
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| Abstract: |
| A capillary electrophoresis（CE）with UV detection method was developed for the determination of ellagic acid and punicalagin in pomegranate peel,grown in different origins.Effects of some important factors,such as pH value,concentration of running buffer,separation voltage and injection time were investigated.The optimum conditions were as the follows:running buffer:50 mmol/L borax buffer（pH 8.7）,separation voltage:20 kV,injection time:9 s/20 kV.Under the optimum conditions,two analytes could be separated within 12 min at the detection wavelength of 260 nm.The good linearities between peak area and mass concentration were obtained in the ranges of 5.0×10-7-5.0×10-5 g/mL and 2.0×10-6-5.0×10-5 g/mL for ellagic acid and punicalagin,with their detection limits of 1.0×10-7g/mL and 1.3×10-6g/mL,respectively.The relative standard deviations（RSD,n=7） for the peak areas of ellagic acid and punicalagin were 2.4% and 2.2%,respectively.The average recoveries were 101% for ellagic acid and 98% for punicalagin.It is found by t-test that there were no significant differences between this method and the HPLC method.The proposed method was simple，rapid and accurate,and was successfully applied in the determination of the analytes in pomegranate peel samples. |
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