| A simple and rapid method was developed for the simultaneous determination of 8 prohibited drugs，including minoxidil,estriol,spironolactone,canrenone,estrone,estradiol,diethylstibestrol and progesterone,in hair growth cosmetics.The sample was extracted by methanol and then purified by an HLB solid phase extraction cartridge.The extraction residue was redissolved in methanol and separated on an Agilent Poroshell 120 Bonus-RP chromatographic column by gradient elution using 10 mmol/L ammonium acetate-methanol as mobile phase.The simultaneous qualitative and quantitative analysis of 8 prohibited drugs were performed by high performance liquid chromatography-diode array detection(HPLC/DAD).Under the optimized conditions,the calibration curves of 8 drugs were linear in the range of 0.5-100 mg/L.The average recoveries of 8 drugs at three spiked levels of 5.0,10.0,50.0 mg/kg were between 80% and 96%,with relative standard deviations of 3.7%-8.9%.The quantitation limits(S/N=10) of spironolactone and progesterone were 2.0 mg/kg,and those of the rest drugs were 5.0 mg/kg.The method was simple,rapid and accurate,and could meet the requirement for the determination of 8 prohibited drugs in hair growth cosmetics. |