Simultaneous Determination of Six Chemical Drugs Illegally Added in Dietary Supplement by High Performance Liquid Chromatography Tandem Mass Spectrometry
  
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DOI:10.3969/j.issn.1004-4957.年份.月份
KeyWord:weight-loss chemical drugs  high performance liquid chromatography-tandem mass spectrum(HPLC-MS/MS)  functional foods
  
AuthorInstitution
黄芳,吴惠勤,黄晓兰,邓欣,罗辉泰,朱志鑫,林晓珊 中国广州分析测试中心广东省分析测试技术公共实验室
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Abstract:
      A high performance liquid chromatography-mass spectrometric(HPLC-MS/MS) method was proposed for the determination of 6 anti-obesity chemical drugs(sibutramine,N-di-desmethyl sibutramine,fenfluramine,phenolphthalein,rimonabant,orlistat)added illegally into health foods.The conditions of extraction procedures and the parameters of HPLC-MS/MS were optimized.The drugs were extracted with methanol,and separated on a C8 chromatographic column using a mixture of acetonitrile and 10 mmol/L ammonium acetate solution as mobile phase with gradient elution.The detection of 6 targeted compounds was performed by positive-ion electrospray ionization mass spectrometry(ESI MS) under multiple reaction monitoring(MRM) mode.Good linearities for 6 compounds were obtained in the range of 0.001-0.5 mg/L with correlation coefficients greater than 0.999.The spiked recoveries were in the range of 81%- 100%with RSDs of 4.8%-6.9%.The detection limits of these drugs were up to 0.5 μg?L-1.The results indicated that this method was simple,rapid and accurate,and was successfully applied in the examination of examine chemical drugs illegally added in the dietary supplement.
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