|
| Simultaneous Determination of Four Tocopherols in Vegetable Oilsby Reverse Phase High Performance Liquid Chromatography |
| |
| View Full Text Download reader |
| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:reverse phase high performance liquid chromatography（RP-HPLC） fluorescence detection tocopherols vegetable oils |
| Author | Institution |
| 于瑞祥，张欣，张秀芹，方晓明，丁卓平 |
1.上海海洋大学食品学院;2.上海出入境检验检疫局 |
|
| Hits: 2873 |
| Download times: 1303 |
| Abstract: |
| A method for the simultaneous determination of four tocopherols in vegetable oils by reverse phase high performance liquid chromatography（RP-HPLC） was established.The sample was extracted with methanol under ultrasonic.The chromatographic separation was carried out on a C30 column（4.6 mm i.d.×250 mm，5 μm） using fluorescence detection at 290 nm of excitation wavelength and 340 nm of emission wavelength.Methanol-water-tert-butyl methyl ether（60∶15∶25）was used as mobile phase by isocratic elution.Results showed that good linear ranges for 4 tocopherols were obtained from 0.050 mg/L to 100.0 mg/L with correlation coefficients（r2）larger than 0.999.The mean recoveries were in the range of 81.3%-93.2% at three spiked levels of 0.50，50.0，500.0 mg/kg，and the relative standard deviations（RSDs） were less than 6%.The limits of detection（LODs） were 0.10 mg/kg for α-tocopherol，0.05 mg/kg for β- and γ-tocopherol，and 0.02 mg/kg for δ-tocopherol. The method is accurate，sensitive and reliable，and could be applied in the real sample analysis. |
| Close |