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| Determination of Erythromycin A and Its Metabolites in Honey by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:honey erythromycin metabolite high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） |
| Author | Institution |
| 张晓燕，郑定钊，袁芳，刘艳，黄娟，许蔚，杨雯筌，丁涛，陈磊，吴斌 |
1.江苏出入境检验检疫局动植物与食品检测中心国家蜂产品基准实验室;2.福建工贸学校 |
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| Abstract: |
| A high performance liquid chromatography-tandem mass spectrometric method for the determination of erythromycin A and its metabolites including erythromycin A enolether and anhydroerythromycin A was developed.The sample was extracted with pH 7.0 phosphate buffer and cleaned-up with HLB column.The detection of target compounds was performed by LC-MS with electrospray positive ionization under the multiple reaction monitoring(MRM) mode.The internal standard method was used for quantitative analysis.Under the optimal conditions,the calibration curves were linear in the range of 2.0-100.0 μg/L with correlation coefficients more than 0.996.The limits of detection and the limits of quantitation were 0.5 μg/kg and 2.0 μg/kg,respectively.The recoveries of three compounds at fortified levels of 2.0,5.0 and 10.0 μg/kg ranged from 70.5% to 109.2% with RSDs less than 10%.The method,with higher recoveries and good precision,was suitable for the determination of erythromycin A and its metabolites in honey. |
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