Simultaneous Determination of Seven Sulfonylurea Hypoglycemic Agents in Health Care Products by Solid Phase Extraction/High Performance Liquid Chromatography
  
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DOI:10.3969/j.issn.1004-4957.年份.月份
KeyWord:solid phase extraction(SPE)  high performance liquid chromatography(HPLC)  health care products  sulfonylurea hypoglycemic agents
  
AuthorInstitution
周春燕,唐柏彬,郗存显,陈冬东,张雷,彭涛,王国民,陈志琼 1.重庆医科大学药学院;2.重庆出入境检验检疫局重庆市进出口食品安全工程技术研究中心;3.中国检验检疫科学研究院
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Abstract:
      A method was established for the simultaneous determination of seven sulfonylurea hypoglycemic agents(e.g.glipizide,tolbutamide,tolazamide,gliclazide,glibenclamide,glimepiride and glurenor) in health care products by solid phase extraction/high performance liquid chromatography(SPE/HPLC).Various samples were extracted with methanol by ultrasound,the extract was cleaned up and concentrated on an LC-C18 SPE column.The chromatographic separation was performed on an Agilent Zorbax SB-C18 column(250 mm×4.6 mm,5 μm) using acetonitrile(A)-0.02% phosphoric acid solution(B) as mobile phase by gradient elution at a flow rate of 1.0 mL/min,a column temperature of 30 ℃ and an ultraviolet(UV) detection wavelength of 228 nm.The seven sulfonylurea hypoglycemic agents showed good linear relationships in the range of 0.2-20 μg/mL with correlation coefficients larger than 0.999 9.The limits of detection(S/N≥3) were 0.1 mg/kg for seven target compounds.The spiked recoveries of seven target compounds in five different health care products ranged from 81% to 115% with relative standard deviations less than 9%.
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