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| Preparation,Characterization of Titanium Dioxide Nanoparticles and Its Application in an Alpha-fetoprotein Immunosensor |
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| DOI：10.3969/j.issn.1004-4957.年份.月份 |
| KeyWord:collagen stencil nano titanium dioxide chitosan gold nanoparticles α-fetoprotein(AFP) immunosensor |
| Author | Institution |
| 朱宇萍，何伟，王碧 |
内江师范学院化学化工学院 |
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| Hits: 2020 |
| Download times: 1002 |
| Abstract: |
| Using pig collagen and titanium sulfate as raw material，by tanning reaction with high temperature calcination，the nanometer titanium dioxide was prepared.Then，fixing the nanometer titanium dioxide-chitosan（Cs） nanocomposites on gold electrodes through electrostatic adsorption，using abundant amino in Cs to absorb gold nano particles and lastly absorb alpha-fetoprotein antibodies,an AFP immunosensor was successfully fabricated.The techniques of UV-vis spectroscopy(UV-Vis)，infrared spectroscopy(FT-IR)，X ray diffraction(XRD) and transmission electron microscopy were used to characterize the nanometer titanium dioxide.Under the optimal conditions，the immune sensor showed a good linear relationship for AFP in the range of 0.01-100 ng/mL，with a correlation coefficient of 0.998 2 and a detection limit of 0.003 ng/mL. |
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